Ruminal fermentation and microbial community differently influenced by four typical subtropical forages in vitro

Animal Nutrition
Muhammad W IqbalBo Lin

Abstract

The present study evaluated the effects of 4 typical subtropical forages on ruminal microbial community composition to formulate a better diet for buffalo. Corn straw silage, elephant grass, cassava residues and sugarcane tail silage were used as substrates for in vitro fermentation. Eight replicates were set up for every substrate, and fermentation was carried out in a 100-mL glass syringe, using buffalo rumen inoculum. Every replicate was anaerobically dispensed with 10 mL of rumen inoculum, 20 mL of McDougall's buffer and 200 mg of dried substrate, and placed in a water bath at 39 °C. Gas production was recorded at 0, 2, 6, 12, 24, 36, 48 and 72 h of incubation. After 24 h, fermentation was ceased for 4 replicates and samples were collected. Volatile fatty acids (VFA) concentrations were measured using gas chromatography. Microbial populations were quantified using quantitative real-time PCR (qRT-PCR), and microbial community was analyzed using high throughput sequencing technology. The results showed, cassava residues as substrate had the highest gas production, acetate, propionate and total VFA concentrations (P < 0.05), and corn straw silage had the lowest acetate:propionate ratio (P < 0.05). The lowest numbers of fungi, ...Continue Reading

Citations

Oct 3, 2020·Microorganisms·Humberto Ramírez-VegaL X Zelaya-Molina
Jul 25, 2019·Journal of Animal Science and Technology·Mahfuzul Islam, Sang-Suk Lee
Aug 23, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Alaa E RabeeSafinaze M Shawket

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BETA
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Excel
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Basic Local Alignment Search Tool ( BLAST )
corrplot
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