Run-down of L-type Ca2+ channels occurs on the alpha 1 subunit

Biochemical and Biophysical Research Communications
L Y HaoMasaki Kameyama

Abstract

Run-down of L-type Ca2+ channels in CHO cells stably expressing alpha 1c, alpha 1c beta 1a, or alpha 1c beta 1a alpha 2 delta gamma subunits was studied using the patch-clamp technique (single channel recording). The channel activity (NPo) of alpha 1c channels was increased 4- and 8-fold by coexpression with beta 1a and beta 1a alpha 2 delta gamma, respectively. When membranes containing channels composed of different subunits were excised into basic internal solution, the channel activity exhibited run-down, the time-course of which was independent of the subunit composition. The run-down was restored by the application of calpastatin (or calpastatin contained in cytoplasmic P-fraction) + H-fraction (a high molecular mass fraction of bovine cardiac cytoplasm) + 3 mM ATP, which has been shown to reverse the run-down in native Ca2+ channels in the guinea-pig heart. The restoration level was 64.7, 63.5, and 66.4% for channels composed of alpha 1c, alpha 1c beta 1a, and alpha 1c beta 1a alpha 2 delta gamma, respectively, and was thus also independent of the subunit composition. We conclude that run-down of L-type Ca2+ channels occurs via the alpha 1 subunit and that the cytoplasmic factors maintaining Ca2+ channel activity act on ...Continue Reading

References

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Citations

Mar 28, 2008·BMC Physiology·Vicenta Salvador-RecatalàRobert M Greenberg
Jun 14, 2002·FEBS Letters·Makoto SuzukiMasashi Imai
Nov 13, 2015·American Journal of Physiology. Cell Physiology·Jianjun XuMasaki Kameyama
Feb 13, 2001·Biochemical and Biophysical Research Communications·L Y HaoM Kameyama
Aug 1, 2006·Biochemical and Biophysical Research Communications·Etsuko MinobeMasaki Kameyama

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