Ru(NH3 )63+ /Ru(NH3 )62+ -Mediated Redox Cycling: Toward Enhanced Triple Signal Amplification for Photoelectrochemical Immunoassay

Analytical Chemistry
Bing WangWei-Wei Zhao

Abstract

Herein we report an effective Ru(NH3)63+/Ru(NH3)62+-mediated photoelectrochemical-chemical-chemical (PECCC) redox cycling amplification (RCA) strategy toward enhanced triple signal amplification for advanced split-type PEC immunoassay application. Specifically, alkaline phosphatase (ALP) label was confined via a sandwich immunorecognition to convert 4-aminophenyl phosphate to the signal reporter 4-aminophenol (AP), which was then directed to interact with Ru(NH3)62+ as a redox mediator and tris (2-carboxyethyl) phosphine (TCEP) as reducing agent in the detection buffer. Upon illumination, the system was then operated upon the oxidation of Ru(NH3)62+ by the photogenerated holes on the Bi2S3/BiVO4 photoelectrode, starting the chain reaction in which the Ru(NH3)62+ was regenerated by Ru(NH3)63+-enabled oxidization of AP to p-quinoneimine, which was simultaneously recovered by TCEP. Exemplified by interleukin-6 (IL-6) as the analyte, the Ru(NH3)63+/Ru(NH3)62+-mediated, AP-involved PECCC RCA coupled with ALP enzymatic amplification could achieve triple signal amplification toward the ultrasensitive PEC IL-6 immunoassay. This protocol can be extended as a general basis for other numerous targets of interest. Besides, we believe this ...Continue Reading

References

Jan 3, 2012·Analytical Chemistry·Md Rajibul AkandaHaesik Yang
Jun 17, 2014·Chemical Reviews·Wei-Wei ZhaoHong-Yuan Chen
Nov 15, 2017·Analytical Chemistry·Wei-Wei ZhaoHong-Yuan Chen
Nov 23, 2017·Analytical Chemistry·Md Rajibul Akanda, Huangxian Ju

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