Running the Titan_ClearLabs Workflow on Terra.bio v1

Frank J AmbrosioTechnical Outreach and Assistance for States Team

Abstract

The Titan_ClearLabs workflow is a part of the Public Health Viral Genomics Titan series for SARS-CoV-2 genomic characterization. Titan_CleanLabs was written to process Clear Labs read data for SARS-CoV-2 ARTIC V3 amplicon sequencing. Upon initiating a Titan_ClearLabs run, input read data provided for each sample will be processed to perform consensus genome assembly, infer the quality of both raw read data and the generated consensus genome, and assign lineage or clade designations as outlined in the Titan_ClearLabs data workflow below. Additional technical documentation for the Titan_ClearLabs workflow is available at: https://public-health-viral-genomics-theiagen.readthedocs.io/en/latest/titan_workflows.html#titan-clearlabs Required input data for Titan_ClearLabs: Cear Labs FASTQ read files (single FASTQ file per sample) Primer sequence coordinates of the PCR scheme utilized in BED file format Video Instruction: Theiagen Genomics: Titan Genomic Characterization https://www.youtube.com/watch?v=zP9I1r6TNrw Theiagen Genomics: Titan Outputs QC https://www.youtube.com/watch?v=Amb-8M71umw For technical assistance, please contact us at: TOAST@cdc.gov

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