Runx2 regulates survivin expression in prostate cancer cells.

Laboratory Investigation; a Journal of Technical Methods and Pathology
Minyoung LimPradip Roy-Burman

Abstract

Previously we described that bone morphogenetic protein-7 (BMP7) could protect prostate cancer C4-2B cells from serum starvation-induced apoptosis via survivin induction. Here, for the first time, we identify Runx2 as a key regulator of survivin transcription. In C4-2B cells grown normally, suppression of Runx2 reduced survivin expression. Using ChIP assays, two regions of the survivin promoter, -1953 to -1812 (I) and -1485 to -1119 (II) encompassing consensus Runx-binding sites were examined. Runx2 was found to be associated with both regions, with a stronger affinity to region-I. In serum-starved cells neither region was occupied, but BMP7 restored association to region-II and not region-I. In reporter assays, transcription activity by BMP7 was significantly reduced when sequences including binding sites of region-II were deleted. Additionally, Runx2 expression was enhanced by BMP7 in these cells. Along with a strong survivin expression, a trend in increased Runx2 expression in human prostate cancer cells and tissues was noted. In the conditional Pten-knockout mouse, Runx2 level increased with growth of prostate tumor. The data define a novel role of Runx2 in regulating survivin expression in malignant epithelial cells and id...Continue Reading

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A J CelesteJ M Wozney
Apr 1, 1995·Molecular and Cellular Biology·S MeyersS W Hiebert
Feb 21, 1998·Nucleic Acids Research·T HeinemeyerN A Kolchanov
Jun 9, 2000·Kidney International·P Ducy, G Karsenty
Apr 5, 2002·Genome Research·W James Kent
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sayyed K ZaidiJane B Lian
Jan 23, 2003·The Prostate·Hiroshi MasudaHidetoshi Yamanaka
Aug 2, 2003·Molecular and Cellular Endocrinology·Liqiang TouJoseph M Alexander
Mar 3, 2004·The Prostate·Hiroshi MasudaHidetoshi Yamanaka
Apr 15, 2005·Development·Jill Wildonger, Richard S Mann
May 3, 2005·Nature Reviews. Cancer·Karen BlythJames C Neil
Apr 15, 2006·The Journal of Biological Chemistry·Eun-Joo JeonSuk-Chul Bae
Dec 14, 2007·Nature Reviews. Cancer·Dario C Altieri
Jan 22, 2008·The Journal of Biological Chemistry·Amjad JavedJane B Lian

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Citations

May 9, 2013·The Journal of Biological Chemistry·Miyuki MatsudaSatoshi Umemura
Dec 22, 2011·Nucleic Acids Research·Gillian H LittleBaruch Frenkel
May 2, 2013·Kidney International·Rafael Kramann, Rebekka K Schneider
Aug 11, 2015·Scientific Reports·Sara Del Mare, Rami I Aqeilan
Jan 5, 2011·Sarcoma·J W MartinJ A Squire
Sep 25, 2010·Molecular Cancer·Sanjeev K BaniwalBaruch Frenkel
Aug 25, 2016·The Journal of Pharmacology and Experimental Therapeutics·Winston VuongJeremy O Jones
Jan 23, 2018·Histochemistry and Cell Biology·Toshihisa Komori
May 30, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Dong-Feng NiuRyohei Katoh
Jul 16, 2011·Hormones & Cancer·Chun-Peng LiaoPradip Roy-Burman
Jul 24, 2014·International Journal of Cancer. Journal International Du Cancer·Xuhui ZhangJane B Lian
Nov 9, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ning ShiShi-You Chen
May 15, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Melinda DuerCatherine M Shanahan
May 6, 2016·American Journal of Respiratory and Critical Care Medicine·Grégoire RuffenachSébastien Bonnet
Aug 10, 2019·Journal of Experimental & Clinical Cancer Research : CR·Gloria ManzottiAlessia Ciarrocchi
May 31, 2018·Molecular Medicine Reports·Gang QuJiahui Yang
Nov 24, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yinhui YangWanhai Xu
Nov 15, 2011·Molecular Cancer Therapeutics·Kunio OkamotoKazuhiko Nakagawa
Mar 15, 2015·Cancer Research·Valentina SancisiAlessia Ciarrocchi
Dec 15, 2020·Open Medicine·Chao HanZhongtao Zhang
Mar 9, 2021·The FEBS Journal·Hannah AsheDaniel Gioeli
Dec 21, 2013·ACS Chemical Biology·Minyoung LimJeremy O Jones

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Methods Mentioned

BETA
gene knockout
transfection
Immunoprecipitation
ChIP
PCR
biopsies
cross-linking ChIP
cross-linking
chromosome shearing
acetylation

Software Mentioned

TF Search
TFsearch
Runx

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