Abstract
The aim of this study is to evaluate the polyphenolic and flavonoid contents in the leaves extracts of Ruscus hypophyllum. Antioxidant activity was estimated by α,α-diphenyl-β-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) assays. The anticoagulant activity of Ruscus extracts was evaluated in vitro, using the prothrombin time (PT) and a PTT-activated partial thromboplastin time tests. The antibacterial activity was tested against large number of important medically and resistant bacteria by the broth dilution method. In this study, ethyl acetate and chloroform extracts displayed the highest total phenols contents (74.76 mg EAG/g and 73.89 mg EAG/g, respectively) and flavonoid content 40 and 32.43 mg EC/g, respectively. The GC-MS analysis of ethyl acetate extract confirmed the presence of oxygenated sesquiterpenes and hydrocarbon diterpenes with percentages of 16.41% and 10.72%, respectively, but chloroform extract was rich with, oxygenated monoterpenes, and oxygenated diterpenes, with percentages of 6.19 and 3.27%, respectively. Among tested extracts, ethyl acetate exhibited the best antioxidant and anticoagulant activities. Furthermore, ethyl acetate and chloroform extracts showed importa...Continue Reading
References
Oct 1, 1995·Archiv der Pharmazie·R M FacinoL Saibene
Mar 1, 1996·Cellular Signalling·C J Masters
Jun 5, 1998·Journal of Ethnopharmacology·M S Ali-ShtayehM A Al-Nuri
Jun 8, 2001·Fitoterapia·E Tuzlaci, P E Aymaz
Jun 29, 2001·The Journal of Pharmacy and Pharmacology·M A FernándezM T Sáenz
Oct 3, 2001·Planta medica·C DemetzosD Angelopoulou
Sep 30, 2005·Journal of Medicinal Chemistry·Christos A Kontogiorgis, Dimitra J Hadjipavlou-Litina
Nov 15, 2008·International Journal of Biological Macromolecules·Wenjun MaoShoudong Guo
Dec 20, 2008·Pharmacology·Pier Carlo BragaMaria Culici
Dec 17, 2009·Bioorganic & Medicinal Chemistry Letters·Dharmendra SaikiaArvind S Negi
May 11, 2010·Thrombosis Research·Hua-Ying FanKe Liu
Aug 10, 2010·NeuroImage·Ranganatha SitaramNiels Birbaumer
Mar 23, 2011·Journal of Ethnopharmacology·Valentina SavoReedy David
May 19, 2011·Pharmacognosy Research·A Vieira
Mar 9, 2012·BMC Complementary and Alternative Medicine·Veeramuthu DuraipandiyanChinnasamy Muthukumar
Jul 4, 2012·Neuroscience Letters·J P CostaR M Freitas
Oct 11, 2013·Journal of Agricultural and Food Chemistry·Ling ZhaoTianfeng Chen
Apr 9, 2014·Carbohydrate Polymers·Yufeng WangYangyang Chen
Jun 11, 2014·Pharmacognosy Magazine·Angelo LuísFernanda Domingues
Oct 23, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ming-Ching ChengTuzz-Ying Song
May 10, 2017·Pharmacognosy Magazine·Luc VerschaeveMaha Mastouri
Jan 20, 2018·Microbiology Spectrum·José Luis Martínez