Ruta graveolens L. induces death of glioblastoma cells and neural progenitors, but not of neurons, via ERK 1/2 and AKT activation

PloS One
Maria Teresa GentileLuca Colucci-D'Amato

Abstract

Glioblastoma multiforme is a highly aggressive brain tumor whose prognosis is very poor. Due to early invasion of brain parenchyma, its complete surgical removal is nearly impossible, and even after aggressive combined treatment (association of surgery and chemo- and radio-therapy) five-year survival is only about 10%. Natural products are sources of novel compounds endowed with therapeutic properties in many human diseases, including cancer. Here, we report that the water extract of Ruta graveolens L., commonly known as rue, induces death in different glioblastoma cell lines (U87MG, C6 and U138) widely used to test novel drugs in preclinical studies. Ruta graveolens' effect was mediated by ERK1/2 and AKT activation, and the inhibition of these pathways, via PD98058 and wortmannin, reverted its antiproliferative activity. Rue extract also affects survival of neural precursor cells (A1) obtained from embryonic mouse CNS. As in the case of glioma cells, rue stimulates the activation of ERK1/2 and AKT in A1 cells, whereas their blockade by pharmacological inhibitors prevents cell death. Interestingly, upon induction of differentiation and cell cycle exit, A1 cells become resistant to rue's noxious effects but not to those of temoz...Continue Reading

References

Oct 1, 1979·Journal of Ethnopharmacology·G A Conway, J C Slocumb
Feb 1, 1992·International Journal of Cancer. Journal International Du Cancer·F O RanellettiM Piantelli
Dec 1, 1988·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·A RehemtullaD A Hart
Jul 26, 1968·Science·P BendaW Sweet
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·D CalifanoV de Franciscis
Oct 21, 1999·Experimental Cell Research·G L Colucci-D'AmatoU di Porzio
Apr 5, 2000·The Journal of Biological Chemistry·D CalifanoV de Franciscis
Jul 28, 2001·Journal of Natural Products·C C ChenJ C Ou
Jul 26, 2002·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·F MirshahiC Soria
Oct 28, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Luca Colucci-D'AmatoUmberto di Porzio
Nov 14, 2003·Reproductive Toxicology·Jorge L Gutiérrez-PajaresJosé Pino
Jan 24, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Martin J van den BentRoger Stupp
Jun 12, 2008·Journal of the National Cancer Institute·Vicki Brower
Sep 12, 2008·Neuro-oncology·James J VredenburghHenry S Friedman
Jul 11, 2009·Science·Jesse W-H Li, John C Vederas
Feb 19, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·M RatheeshA Helen
Jan 5, 2011·Frontiers in Bioscience (Landmark Edition)·Ewa Brzezianska, Dorota Pastuszak-Lewandoska
Jul 26, 2011·Annals of Neurology·Matthias PreusserRoger Stupp
Oct 11, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yi JingBing-Hua Jiang
Dec 24, 2011·Cancer Science·Wenzhuo ZhuangZhongqin Liang
Jan 3, 2012·European Journal of Medicinal Chemistry·Tânia R MielckeMaria M Campos
Jun 5, 2012·Journal of Neuro-oncology·Ági OszvaldVolker Seifert
Sep 11, 2012·Journal of Neurochemistry·Maria Teresa GentileLuca Colucci-D'Amato
Oct 23, 2012·Science·Dinorah Friedmann-MorvinskiInder M Verma
Jan 25, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·M RatheeshAntony Helen
May 15, 2013·Journal of Neuro-oncology·Antonio RuggieroRiccardo Riccardi

❮ Previous
Next ❯

Citations

Jul 22, 2017·Drug Testing and Analysis·Tiziana NardinRoberto Larcher
May 31, 2019·Cancers·Olga PastorinoLuca Colucci-D'Amato
Jul 31, 2019·Phytotherapy Research : PTR·Moumita KunduMahitosh Mandal
Nov 24, 2016·Molecular Medicine Reports·Yingge WangJingyan Liang
Sep 9, 2017·Journal of Cellular Physiology·Tiziana SquillaroMariarosa A B Melone
Aug 18, 2018·Molecular Neurobiology·J H AzambujaElizandra Braganhol
Mar 15, 2019·CNS & Neurological Disorders Drug Targets·Carlos Tadeu Parisi de OliveiraDenise Gonçalves Priolli
Nov 29, 2017·BioMed Research International·Moon Nyeo ParkBonglee Kim
Jan 12, 2021·Current Medical Science·Fahad Hassan ShahBashir Ahmad
Jan 28, 2021·International Journal of Molecular Sciences·Mafalda Giovanna RecciaLuca Colucci-D'Amato
Oct 25, 2020·International Journal of Molecular Sciences·Luca Colucci-D'AmatoFloriana Volpicelli
Jun 14, 2020·Journal of Ethnopharmacology·Alexandra T CoimbraAna Paula Duarte
Oct 16, 2020·Critical Reviews in Food Science and Nutrition·Arakkaveettil Kabeer FarhaHarold Corke
May 3, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mariana MagalhãesCélia Cabral

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
scraping

Software Mentioned

Scion Image
Tali

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Cancer Stem Cells in Glioblastoma

Glioblastoma is the most common and aggressive type of brain tumor. It contains a population of tumor initiating stem cell-like cells known as cancer stem cells. Investigations are ongoing into these cancer stem cells found in these solid tumors which are highly resistance to treatment. Here is the latest research on cancer stem cells in glioblastoma.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis