Abstract
Gap junctions, which is formed by connexins, has been proved to play an important role in the atherogenesis development. Rutaecarpine was reported to inhibited monocyte migration, which indicates its potential for anti-atherosclerosis activity. This study evaluated the effect of rutaecarpine on endothelial dysfunction, and focused on the regulation of connexin expression in endothelial cells by rutaecarpine. Endothelia damage was induced by exposing HUVEC-12 to Ox-LDL (100mg/l) for 24h, which decreased the expression of protective proteins Cx37 and Cx40, but induced atherogenic Cx43 expression, in both mRNA and protein levels, concomitant with the impaired propidium iodide diffusion through the gap junctions. Pretreatment with rutaecarpine effectively recovered the expression of Cx37 and Cx40, but inhibited Cx43 expression, thereby improving gap junction communication and significantly prevented the endothelial dysfunction. Consequently, the cell viability and nitric oxide production were increased, lactate dehydrogenase production was decreased and monocyte adhesion was inhibited. These protective effects of rutaecarpine were remarkably attenuated by pretreatment with capsazepine, a competitive antagonist of transient receptor...Continue Reading
References
Jun 9, 1998·Cytokine·H V van RijenH J Jongsma
May 11, 2001·Planta medica·Y KobayashiT Kamiya
Sep 11, 2002·Planta medica·Chang-Ping HuYuan-Jian Li
Jan 15, 2003·Journal of Leukocyte Biology·Stefan ZahlerUlrich Pohl
Feb 26, 2003·Circulation·Brenda R KwakFrançois Mach
Mar 8, 2003·Planta medica·Chang-Ping HuYuan-Jian Li
Jun 17, 2004·Circulation·Jean Davignon, Peter Ganz
Jul 25, 2006·Nature Medicine·Cindy W WongBrenda R Kwak
Jul 1, 2008·Regulatory Peptides·Dan LuoYuan-Jian Li
Oct 7, 2008·Antioxidants & Redox Signaling·Anne C BrissetBrenda R Kwak
Feb 7, 2009·Physiology·Laurent BurnierBrenda R Kwak
Feb 26, 2009·Journal of Cellular Biochemistry·Sook-Kyoung HeoSun-Dong Park
Mar 4, 2009·Bioorganic & Medicinal Chemistry·Zhuo ChenYe Xie
Apr 7, 2009·Atherosclerosis·Jean-Paul DerouetteBrenda R Kwak
May 1, 2009·Seminars in Immunopathology·Sandrine MorelBrenda R Kwak
Oct 27, 2009·Toxicology·Eun Hee HanHye Gwang Jeong
Dec 23, 2009·Circulation·C E ChadjichristosB R Kwak
Mar 26, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sujie Jia, Changping Hu
Oct 22, 2010·Canadian Journal of Physiology and Pharmacology·Jian-Zhe LiYuan-Jian Li
Feb 16, 2011·Chinese Medicine·Jyh-Fei LiaoChieh-Fu Chen
May 31, 2011·Cell Calcium·Elke DecrockLuc Leybaert
Aug 13, 2011·Biochimica Et Biophysica Acta·R C Looft-WilsonB E Isakson
Mar 27, 2012·Biochemical and Biophysical Research Communications·Dongdong YuanAndrew L Harris
May 23, 2012·Biochimica Et Biophysica Acta·Anna PfennigerBrenda R Kwak
Jun 5, 2012·Journal of Molecular and Cellular Cardiology·Anna PfennigerBrenda R Kwak
Apr 10, 2013·Pflügers Archiv : European journal of physiology·Eno E Ebong, Natacha Depaola
Jun 19, 2013·Chinese Journal of Integrative Medicine·Yan-ju LiAn-sheng Sun
Citations
Nov 3, 2016·Scientific Reports·Mohammad A AlamTianhong Yang
Mar 16, 2018·International Journal of Molecular Sciences·Robert D Johnson, Patrizia Camelliti
Feb 28, 2018·Medicinal Research Reviews·Xiao-Fei ShangKuo-Hsiung Lee
Apr 3, 2019·Journal of Cellular Biochemistry·Arun Bahadur GurungAtanu Bhattacharjee
May 31, 2020·Cells·Sharon NegriFrancesco Moccia
Jan 12, 2017·Molecular Medicine Reports·Mei Tang, Jun Fang
Feb 10, 2016·Journal of Cardiovascular Pharmacology·Yong LiuDan Luo
Dec 3, 2020·Frontiers in Cell and Developmental Biology·Jia Zheng, Chengzhi Lu