Ruthenium rad staining and tannic acid fixation of dental basement membrane

Cell and Tissue Research
J M MeyerJ V Ruch

Abstract

Ruthenium red staining and tannic acid fixation were used to analyse the fine structure of embryonic mouse dental basement membrane in intact first mandibular molars or in EDTA-isolated dental papillae. Preameloblasts are separated from extracellular matrix proper by a basal lamina that contains regularly arranged proteoglycan granules of about 10 nm in diameter. This distribution pattern is particularly evident in the inner and outer lamina rara of the basal lamina associated with EDTA-isolated dental papillae. The plasmalemma of preameloblasts demonstrates electron dense plaques on the inner leaflet. Ruthenium red positive granules (50 nm in diameter) coat nonstriated and striated fibrils of the matrix. Hyaluronidase treatment digested the ruthenium red positive granules. Tannic acid fixation allowed the demonstration of filaments within the lamina rara interna, connecting the lamina densa with plasmalemma of preameloblasts. These observations are discussed in the context of the terminal differentiation of odontoblasts.

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Citations

Jan 1, 1986·Progress in Histochemistry and Cytochemistry·E J Sanders
Jan 1, 1987·Progress in Histochemistry and Cytochemistry·M GoldbergF Escaig-Haye
Jan 1, 1988·Archives of Oral Biology·D TziafasJ V Ruch
Sep 1, 1981·Cell and Tissue Kinetics·J V Neal, C S Potten
Jan 1, 1985·Differentiation; Research in Biological Diversity·H LesotJ V Ruch
Jan 1, 1982·Differentiation; Research in Biological Diversity·J V RuchM Olive
May 27, 2015·PloS One·Hana DosedělováMarcela Buchtová
Apr 1, 1985·Journal of Dental Research·J V Ruch
Jul 1, 1987·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Colin A B JahodaPhilippe Sengel
Nov 1, 1990·Ultrastructural Pathology·K P Dingemans, M A van den Bergh Weerman
Jul 1, 1988·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Marie-Dominique KublerJean Victor Ruch

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