Ruthenium red as an effective blocker of calcium and sodium currents in guinea-pig isolated ventricular heart cells

British Journal of Pharmacology
Claire O MalécotJorge A Argibay

Abstract

1. The effect of ruthenium red on calcium and sodium currents was studied in guinea-pig isolated ventricular heart cells with the whole cell patch-clamp technique. 2. Ruthenium red very efficiently blocked the L-type calcium current in a dose-dependent manner. A significant block was observed for concentrations as low as 0.3 microM. Analysis of the dose-response curve with the logistic equation indicated an EC50 of 0.8 microM, a maximum inhibition of 85% reached at 5 microM, and a coefficient of 2.37. 3. There was no shift in the voltage-dependence of the Ca current activation, nor in that of its steady-state inactivation determined with a 1 s prepulse. However, removal of Ca current inactivation at positive voltage was considerably reduced in the presence of concentrations of ruthenium red above 1 microM. A slowing of the time-course of inactivation of the Ca current was also observed. 4. At 10 microM, a concentration generally used to block the sarcoplasmic Ca release channels or the mitochondrial Ca uptake, ruthenium red blocked 26.7+/-4.3% (n=8) of the sodium current, and slowed its inactivation time-course. No effect was observed on the voltage-dependence of the current activation or inactivation. The peak sodium current w...Continue Reading

References

Sep 1, 1979·Cell and Tissue Research·M S Forbes, N Sperelakis
Nov 1, 1977·The Journal of General Physiology·F Bezanilla, C M Armstrong
Mar 1, 1992·Pflügers Archiv : European journal of physiology·Y Zhu, T M Nosek
May 1, 1991·The Journal of Membrane Biology·M Kohlhardt
May 31, 1990·Biochemical and Biophysical Research Communications·H MasuokaT Tanaka
Nov 1, 1990·Journal of Cardiovascular Pharmacology·G J GroverP G Sleph
Apr 11, 1991·Cardiovascular Research·V M FigueredoA M Keller
Oct 1, 1991·Pflügers Archiv : European journal of physiology·A V ZholosM F Shuba
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E W McCleskey, W Almers
Apr 1, 1987·Canadian Journal of Physiology and Pharmacology·J O Bustamante
Jun 1, 1989·The American Journal of Physiology·K OnoM Arita
Sep 1, 1987·The Biochemical Journal·R RizzutoG F Azzone
Jan 22, 1971·Biochemical and Biophysical Research Communications·C L Moore
Jan 21, 1972·Biochimica Et Biophysica Acta·F D VasingtonE Carafoli
Oct 1, 1982·The Journal of General Physiology·J R Stimers, L Byerly
May 6, 1984·Nature·P Hess, R W Tsien
Oct 1, 1993·Pflügers Archiv : European journal of physiology·D FeldmeyerP Zöllner
Jan 20, 1994·Biochemical Pharmacology·A GomisJ A Reig
Jan 1, 1994·Pflügers Archiv : European journal of physiology·H MuramatsuH Hidaka
Jan 1, 1995·Fundamental & Clinical Pharmacology·A LeperreB K Keppler

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Jan 5, 2000·Physiological Reviews·D G Nicholls, S L Budd
May 23, 2018·International Journal of Molecular Sciences·Kjell De VrieseSteffen Vanneste
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Max W G RobertsChanghao Wu
Feb 5, 2002·Journal of Neurophysiology·Guang Jian Wang, Stanley A Thayer
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