Ruthenium red-catalyzed degradation of peroxides can prevent mitochondrial oxidative damage induced by either tert-butyl hydroperoxide or inorganic phosphate

Archives of Biochemistry and Biophysics
A R MeinickeA E Vercesi

Abstract

We have recently shown that ruthenium red, a non-competitive inhibitor of the mitochondrial Ca2+ uniporter, can reduce tert-butyl hydroperoxide via a Fenton-type reaction. In respiring mitochondrial preparations containing tert-butyl hydroperoxide, redox cycling of ruthenium red occurs and causes the amplification of methyl radical generation (Meinicke, A. R., Zavan, S. S., Ferreira, A. M. C., Vercesi, A. E., and Bechara, E. J. H. (1996) Arch. Biochem. Biophys. 328, 239-244). In this study we show that ruthenium red can act as an antioxidant preventing mitochondrial damage when the respiratory chain is reduced or when ascorbate is present. Ruthenium red can catalyze the degradation of hydrogen peroxide into H2O and O2. We show here that ruthenium red prevents both accumulation of mitochondrial generated H2O2 and swelling in the presence of the Ca2+ ionophore A23187. Under these conditions the damage induced by Ca2+ ions and either tert-butyl hydroperoxide or inorganic phosphate is promoted by mitochondrial-generated reactive oxygen species. Swelling induced by phenylarsine oxide, a thiol cross-linker, by a mechanism independent of free radicals is not inhibited by ruthenium red. These data provide evidence that the antioxidant ...Continue Reading

References

Sep 1, 1979·Cell and Tissue Research·M S Forbes, N Sperelakis
Jan 1, 1978·Methods in Enzymology·J A Buege, S D Aust
May 1, 1990·The American Journal of Physiology·T E Gunter, D R Pfeiffer
Jan 1, 1989·Free Radical Biology & Medicine·H C Sutton, C C Winterbourn
Aug 15, 1988·Biochemical and Biophysical Research Communications·A E VercesiG Fiskum
Jan 22, 1971·Biochemical and Biophysical Research Communications·C L Moore
Apr 15, 1996·Archives of Biochemistry and Biophysics·A R MeinickeE J Bechara
Jan 1, 1996·Free Radical Biology & Medicine·F AntunesR E Pinto

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Citations

Nov 22, 2005·Chemistry & Biology·Adrian IsraelsonVarda Shoshan-Barmatz
Jan 5, 2000·Physiological Reviews·D G Nicholls, S L Budd
Jan 25, 2012·Basic & Clinical Pharmacology & Toxicology·Ian C WinburnJoanne C Harrison

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