Ruthenium red effect on mechanical and electrical properties of mammalian skeletal muscle.

Life Sciences
O Delbono, B A Kotsias

Abstract

In this work we studied the effect of ruthenium red (RR) on the mechanical and electrical properties of rat diaphragm bundles in vitro (30 degrees C). Two concentrations of RR were used: 5 and 10 microM. We measured: 1) twitches, tetanus and caffeine contracture; 2) relation between mechanical tension and resting membrane potential (Vm); 3) contraction threshold by visualization of the contraction around the stimulated area of the fiber. The main finding are the following: a) RR potentiates the twitch tension. The tetanic tension is not affected and the time course of the caffeine contracture is shortened in the RR containing solutions; b) the relationship between mechanical tension and resting membrane potential (Vm) is shifted toward more negative values of Vm in RR; c) the mechanical threshold is lowered about 7 mV in the presence of RR; d) the rates of depolarization and repolarization of the action potential are decreased in the test solutions. We suggested that the shift in the mechanical threshold and the prolongation of the action potential are the main factors involved in the potentiating effect of RR. The mechanism by which RR shifts the mechanical threshold is not known.

References

Jun 12, 1979·Pflügers Archiv : European journal of physiology·M Dörrscheidt-Käfer
Jun 12, 1979·Pflügers Archiv : European journal of physiology·M Dörrscheidt-Käfer
Feb 1, 1987·Pflügers Archiv : European journal of physiology·C Léoty, J Noireaud
Jan 1, 1986·The American Journal of Physiology·B A KotsiasC A Obejero Paz
Jan 1, 1974·The Journal of Membrane Biology·V M Madeira, M C Antunes-Madeira
Jul 1, 1974·The Journal of Cell Biology·J N Howell
Jan 22, 1971·Biochemical and Biophysical Research Communications·C L Moore
Oct 1, 1971·The American Journal of Physiology·H Lorković
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R Rahamimoff, E Alnaes
Feb 1, 1968·The Journal of General Physiology·B Hille
Aug 1, 1984·Journal of Muscle Research and Cell Motility·K W Snowdowne, J N Howell
Jan 1, 1984·Pflügers Archiv : European journal of physiology·J Y Su, W Hasselbach
Apr 1, 1982·Canadian Journal of Physiology and Pharmacology·G Cota, E Stefani
Sep 1, 1960·The Journal of Physiology·A L HODGKIN, P HOROWICZ

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.