RUTHENIUM RED INHIBITION OF OXYGEN EVOLUTION AND SPECIFIC RELEASE OF THE EXTRINSIC 16 kDa POLYPEPTIDE IN A PHOTOSYSTEM II PREPARATION

Photochemistry and Photobiology
Paul JursinicR Carpentier

Abstract

The inhibitory effect of the dye ruthenium red was studied in photosystem II-enriched submembrane fractions. A number of distinct types of interaction were found, which differed in their concentration range and required incubation time. Ruthenium red instantaneously quenches the initial chlorophyll a fluorescence level (F0) and the maximum fluorescence level (Fm) by enhancing radiationless deactivation in the chlorophyll light harvesting complex. Associated with this quenching of fluorescence is an instantaneous decrease in the quantum yield of oxygen evolution. Ruthenium red also inhibited the light saturated rate of oxygen evolution and the variable fluorescence, monitored 80 µs after a saturating excitation-flash. These inhibitions increased with incubation time and became greater than 50% within 5 min. Although ruthenium red was known to affect Ca2+or Cl-sites specifically, the inhibitory action was more pronounced than simple Ca2+or Cl-depletion. Incubation with ruthenium red for 5 min blocks the Z P680+→ Z+P680 charge transfer reaction. Upon mixing with the photosystem II preparation, ruthenium red induced specific release of the extrinsic 16 kDa polypeptide associated with water-splitting without release of Mn. It is pro...Continue Reading

References

Jan 31, 1975·Biochimica Et Biophysica Acta·W L Butler, M Kitajima
Jun 23, 1972·Biochimica Et Biophysica Acta·S Okayama, W L Butler
Dec 1, 1968·Plant Physiology·T Yamashita, W L Butler
Jun 15, 1984·Biochemical and Biophysical Research Communications·H Y Nakatani
Apr 16, 1984·Biochemical and Biophysical Research Communications·H Y Nakatani
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·O Nanba, K Satoh
Feb 22, 1974·Biochimica Et Biophysica Acta·A L EtienneJ Lavorel

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Citations

Sep 19, 2008·Luminescence : the Journal of Biological and Chemical Luminescence·Alain Gauthier, Robert Carpentier
Oct 19, 2004·Journal of Photochemistry and Photobiology. B, Biology·Ariadna Garza-OrtizBlas Lotina-Hennsen

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