Rv0132c of Mycobacterium tuberculosis encodes a coenzyme F420-dependent hydroxymycolic acid dehydrogenase

PloS One
Endang Purwantini, Biswarup Mukhopadhyay

Abstract

The ability of Mycobacterium tuberculosis to manipulate and evade human immune system is in part due to its extraordinarily complex cell wall. One of the key components of this cell wall is a family of lipids called mycolic acids. Oxygenation of mycolic acids generating methoxy- and ketomycolic acids enhances the pathogenic attributes of M. tuberculosis. Thus, the respective enzymes are of interest in the research on mycobacteria. The generation of methoxy- and ketomycolic acids proceeds through intermediary formation of hydroxymycolic acids. While the methyl transferase that generates methoxymycolic acids from hydroxymycolic acids is known, hydroxymycolic acids dehydrogenase that oxidizes hydroxymycolic acids to ketomycolic acids has been elusive. We found that hydroxymycolic acid dehydrogenase is encoded by the rv0132c gene and the enzyme utilizes F420, a deazaflavin coenzyme, as electron carrier, and accordingly we called it F420-dependent hydroxymycolic acid dehydrogenase. This is the first report on the involvement of F420 in the synthesis of a mycobacterial cell envelope. Also, F420-dependent hydroxymycolic acid dehydrogenase was inhibited by PA-824, and therefore, it is a previously unknown target for this new tuberculos...Continue Reading

References

Jul 25, 1976·Journal of Molecular Biology·C Chothia
May 30, 1975·Molecular and Cellular Biochemistry·E LedererJ Wietzerbin
Jan 1, 1990·Annual Review of Biochemistry·A A DiMarcoR S Wolfe
Jan 1, 1972·Progress in Biophysics and Molecular Biology·A A Zamyatnin
Oct 1, 1984·Archives of Microbiology·D E MinnikinM Magnusson
Jan 1, 1995·Annual Review of Biochemistry·P J Brennan, H Nikaido
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y Yuan, C E Barry
Nov 5, 1997·Advances in Microbial Physiology·M Daffé, P Draper
Jan 28, 1999·Methods in Molecular Biology·G S Besra
Jan 28, 1999·Methods in Molecular Biology·P O Gaora
Nov 28, 2000·The Journal of Biological Chemistry·M S GlickmanW R Jacobs
Mar 21, 2002·Expert Opinion on Therapeutic Targets·Shampa KhasnobisDelphi Chatterjee
Apr 30, 2003·The Journal of Biological Chemistry·Tetsuya YagiPatrick J Brennan
Jan 18, 2005·Clinical Microbiology Reviews·Kuni TakayamaGurdyal S Besra
Jan 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ujjini H ManjunathaClifton E Barry
Mar 23, 2006·Theoretical Biology & Medical Modelling·J C Biro
Mar 28, 2006·The American Journal of Pathology·Robert L HunterJeffrey K Actor
Mar 10, 2007·Current Topics in Medicinal Chemistry·Patrick J Brennan, Dean C Crick
Mar 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Endang Purwantini, Biswarup Mukhopadhyay
Jul 31, 2009·Communicative & Integrative Biology·Ujjini ManjunathaClifton E Barry

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Citations

Oct 11, 2014·Protein Science : a Publication of the Protein Society·Ghader Bashiri, Edward N Baker
Dec 15, 2016·Applied Microbiology and Biotechnology·Quoc-Thai NguyenMarco W Fraaije
Feb 24, 2018·The Journal of Biological Chemistry·Jan MadackiJana Korduláková
May 18, 2016·Journal of Bacteriology·Endang PurwantiniBiswarup Mukhopadhyay
Apr 29, 2016·Microbiology and Molecular Biology Reviews : MMBR·Chris GreeningColin J Jackson
Sep 18, 2016·Applied and Environmental Microbiology·Thanavit JirapanjawatChris Greening
Oct 30, 2016·The Journal of Biological Chemistry·Dwi SusantiBiswarup Mukhopadhyay
Oct 13, 2017·Frontiers in Microbiology·Blair NeyChris Greening
Dec 6, 2018·Scientific Reports·M Laura MascottiMarco W Fraaije
Apr 15, 2021·FEMS Microbiology Reviews·Rhys Grinter, Chris Greening
Jul 9, 2021·Drug Design, Development and Therapy·Stephani L StancilSusan M Abdel-Rahman
Aug 6, 2021·Molecular Biology Reports·Sadhana Sundararajan, Rajiniraja Muniyan

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Methods Mentioned

BETA
NMR
X-ray
environmental stresses
PCR

Software Mentioned

ClustalW

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