RXR agonists inhibit high-glucose-induced oxidative stress by repressing PKC activity in human endothelial cells

Free Radical Biology & Medicine
Dajun ChaiBen He

Abstract

Activation of retinoid X receptor (RXR) is known to exert antiatherogenic effects. However, the underlying mechanism remains unclear. In this study, we examined the effects of the RXR agonists 9-cis-retinoic acid and SR11237 on high-glucose-induced oxidative stress in human endothelial cells. Our results demonstrated that high-glucose-induced oxidative stress in human umbilical vein endothelial cells (HUVECs) was mainly mediated through its activation of the Nox4, gp91phox, and p22phox components of nicotinamide adenine dinucleotide phosphate oxidase. Treatment of endothelial cells with RXR agonists resulted in significant inhibition of high-glucose-induced oxidative stress and expression of Nox4, gp91phox, and p22phox. The effect of RXR agonists was due to their inhibition of Rac-1 activation. Furthermore, RXR agonists rapidly inhibited high-glucose-induced activation of protein kinase C (PKC), an upstream activator of Rac-1. To study whether the rapid inhibitory effects of RXR agonists were mediated by RXR, we examined the effect of RXR downregulation by RXR siRNA. Our results showed that expression of RXR siRNA largely abrogated the effects of RXR agonists, suggesting the requirement of RXR expression. Interestingly, RXRalph...Continue Reading

References

Dec 15, 1995·Cell·D J Mangelsdorf, R M Evans
Jul 6, 2000·Nature Cell Biology·Y KatagiriG Guroff
Feb 7, 2001·Circulation Research·U HinkT Munzel
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·T ClaudelJ Auwerx
May 10, 2002·The Journal of Biological Chemistry·Goran BoskovicRichard M Niles
Nov 12, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Thomas MünzelDavid G Harrison
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Toyoshi InoguchiHajime Nawata
Sep 6, 2003·American Journal of Physiology. Cell Physiology·David GreggPascal J Goldschmidt-Clermont
Jan 14, 2004·Circulation·Tetsuro AgoMitsuo Iida
Oct 13, 2004·Mutation Research·Richard M Niles
Oct 16, 2004·Current Atherosclerosis Reports·Trevor A Mori, Lawrence J Beilin
Dec 21, 2004·Cell Death and Differentiation·A SzantoL Nagy
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Siva Kumar KolluriXiao-kun Zhang
Dec 17, 2005·Circulation Research·Carmine VecchioneGiuseppe Lembo
Sep 12, 2006·Journal of Molecular and Cellular Cardiology·Go HaraguchiMitsuaki Isobe
Sep 30, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Fanny LalloyerAnne Tailleux
Oct 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marika MassaroRaffaele De Caterina
Mar 27, 2007·Nature Reviews. Cancer·Erin M Griner, Marcelo G Kazanietz

❮ Previous
Next ❯

Citations

Dec 20, 2011·Genetics and Molecular Research : GMR·C-H HsiehF-C Hsiao
Feb 11, 2011·Kidney International·Jeffrey L Barnes, Yves Gorin
Aug 30, 2008·Biochemical and Biophysical Research Communications·Peiren ShanBen He
Jul 19, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tomonori AoyamaDavid A Brenner
Jun 19, 2013·Clinical Science·Yves Gorin, Karen Block
Mar 27, 2013·Free Radical Biology & Medicine·Yves Gorin, Karen Block
Mar 9, 2010·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Robert Ringseis, Klaus Eder
Aug 4, 2011·Biochimica Et Biophysica Acta·Eun-Jung RheeJorge Plutzky
May 15, 2012·Mutation Research·Urbain Weyemi, Corinne Dupuy
Oct 13, 2016·Molecular Biology Reports·Petra PriščákováVanda Repiská
Apr 24, 2018·Acta Ophthalmologica·Suzanne BroadgateVictor Chong
Apr 26, 2019·Anti-cancer Drugs·Rana Abdel-SamadNadine Darwiche
Aug 24, 2017·The American Journal of Chinese Medicine·Li-Yen HuangShih-Yu Lee
May 30, 2014·American Journal of Physiology. Renal Physiology·Nagaraj ManickamJeffrey L Barnes
Nov 18, 2014·Current Opinion in Nephrology and Hypertension·Vicki Thallas-BonkeMark E Cooper
Jun 5, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria-Jesus SanzLaura Piqueras
Aug 11, 2010·Journal of Natural Products·Hitoshi KotaniMakoto Inoue
Aug 27, 2021·Cellular Signalling·Alexis Paulina Jiménez-UribeJosé Pedraza-Chaverri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.