Abstract
To assess the anti-angiogenesis potential and mechanism of RY10-4, a derivative of protoapigenone, which was verified the broad-spectrum anti-tumor activities by previous study. RY10-4 and RY10-3 were synthesized according to the procedure described. Breast cancer cells MCF-7 and MDA-MB-231 that got the best performance in the previous anti-tumor activity screening were selected for further anti-cancer mechanism research. Firstly, cells proliferation assay of RY10-4 and RY10-3 was used to demonstrate the fact that the 4-hydroxy-2,5-cyclohexadien-1-one system would be the efficient pharmacophore of RY10-4. Then, a series of assays such as human umbilical vein endothelial cells (HUVECs) proliferation assay, HUVECs migration, tube network formation and morphological observations of zebrafish were applied to confirm its anti-angiogenesis activity. Upon RY10-4 treatment, the HIF-1α and VEGF were analyzed by western blot in normoxic and hypoxic conditions, meanwhile the PI3K-AKT-mTOR pathway-related protein such as AKT, p-AKT, mTOR and p-mTOR was also analyzed. In the MCF-7, MDA-MB-231 and HUVECs proliferation assay, RY10-4 that has 4-hydroxy-2,5-cyclohexadien-1-one system showed distinct advantage compared with RY10-3. Tests had ver...Continue Reading
References
Jan 3, 1990·Journal of the National Cancer Institute·J Folkman
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Jan 1, 1982·Cancer Chemotherapy and Pharmacology·H J Senn
Jan 1, 1995·Nature Medicine·J Folkman
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·S ParangiD Hanahan
Jun 1, 1996·Cancer Metastasis Reviews·K P Claffey, G S Robinson
May 1, 1997·Lancet·A L Harris
Jun 18, 1998·Cell·G D YancopoulosJ Folkman
May 23, 2000·Critical Reviews in Biochemistry and Molecular Biology·G L Semenza
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·M S NeshatC L Sawyers
Feb 2, 2002·Experimental Hematology·Nabanita S Datta, Michael W Long
Nov 26, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohammad Atiqur Rahman, Masakazu Toi
May 4, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G Taraboletti, R Giavazzi
Oct 12, 2004·Biochemical and Biophysical Research Communications·Xiao-Song ZhongBing-Hua Jiang
May 4, 2005·Cancer Research·Tracey D BradshawMalcolm F G Stevens
Jul 25, 2006·Bioorganic & Medicinal Chemistry·Bulbul PanditPui-Kai Li
Mar 21, 2007·Cancer Chemotherapy and Pharmacology·R Longo, G Gasparini
May 16, 2007·Cancer Letters·Dong Hoon ShinJong-Wan Park
Jul 12, 2007·Journal of Medicinal Chemistry·An-Shen LinKuo-Hsiung Lee
Nov 21, 2007·FEBS Letters·Chun-Te ChiangJen-Kun Lin
Mar 14, 2008·The Journal of Pharmacology and Experimental Therapeutics·Hsueh-Ling ChangShyng-Shiou F Yuan
Aug 26, 2009·Biochimie·Bogdan ManolescuCornelia Cercasov
Apr 13, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·X H HuangJ L Ruan
Aug 6, 2010·Investigational New Drugs·Wen-Ying ChenChin-Chung Wu
Jan 19, 2011·European Journal of Pharmacology·Huibin LiuJinlan Ruan
Apr 26, 2011·Bioorganic & Medicinal Chemistry Letters·Qianying YuanJinlan Ruan
Jun 28, 2011·Free Radical Biology & Medicine·Giuseppina ComitoPaola Chiarugi
Jul 12, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Anhua WeiJinlan Ruan
Jul 16, 2011·Cancer Letters·Tzu-Chao ChuangVinchi Wang
Jul 22, 2011·The Breast : Official Journal of the European Society of Mastology·Susumu ShibasakiMasato Takahashi
Dec 17, 2011·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Dong LiuZhaocong Chen
Dec 20, 2011·Neuroscience Letters·Hongju ChenDezhi Mu
Citations
Dec 3, 2013·Archives of Toxicology·Ching-Hao LiJaw-Jou Kang
Jul 23, 2013·Cellular and Molecular Life Sciences : CMLS·Inna M YasinskaVadim V Sumbayev
Jul 23, 2015·Cancer Chemotherapy and Pharmacology·Tijana StankovićMilica Pešić
Mar 15, 2016·PloS One·Xuenong ZhangJinlan Ruan
Oct 20, 2018·Anti-cancer Drugs·Chuanlong GuoDayong Shi