Mar 1, 1997

Ryanodine receptor binding constants in skeletal muscle, heart, brain and liver of the Mexican volcano mouse (Neotomodon alstoni alstoni; Rodentia:Cricetidae). Comparison with five other rodent species

The International Journal of Biochemistry & Cell Biology
T Martínez-MerlosM Diaz-Muñoz


Equilibrium [3H]ryanodine binding assay was applied to total membrane fractions of six rodent species, including the Mexican volcano mouse Neotomodon alstoni alstoni, Wistar rat Rattus norvegicus albinus, golden hamster Mesocritus auratus, gerbil Meriones unguiculatus, guinea-pig Cavia porcellus, and ground squirrel Spermophillus mexicanus. The organs selected for this study were: skeletal muscle, heart, brain and liver. The constants derived from Scatchard analysis show slight variations in their Kd, ranging from 3 to 15 nM, except in the gerbil's skeletal muscle (38 nM) and the hamster's brain (27 nM). Remarkably, the Bmax calculated in guinea-pig muscle was as high as that reported for the rabbit fast twitch muscle (4.6 pmol/mg of protein) using the same membrane fraction preparation. For all the other skeletal muscles, Bmax was similar to the corresponding heart Bmax values (from 0.5 to 1 pmol/mg of protein). Gerbil cardiac Bmax was the highest (1.1 pmol/mg of protein). The ground squirrel was the rodent with more cerebral ryanodine binding sites (0.26 pmol/mg of protein), whereas the rat and the volcano mouse showed the lowest values (0.12 pmol/mg of protein). The richest sources of hepatic ryanodine receptor were the guin...Continue Reading

Mentioned in this Paper

Neotomodon alstoni
Mesocricetus auratus
Cytochrome c Oxidase Subunit VIa
Ryanodine Receptor 3
Membrane Fraction
Smooth Endoplasmic Reticulum

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