Ryanodine receptor expression in the kidney and a non-excitable kidney epithelial cell.

The Journal of Biological Chemistry
R E Tunwell, F A Lai

Abstract

An oligonucleotide probe to a conserved 3' region within the three identified ryanodine receptor-calcium release channel isoforms hybridized to a single clone from a rabbit kidney cDNA library. The kidney clone encoded the carboxyl-terminal 338 amino acids within the putative transmembrane domain of the type 2 ryanodine receptor sequence. Reverse transcriptase-polymerase chain reaction with isoform-specific oligonucleotide primers demonstrated the presence of the type 2 ryanodine receptor transcript in rabbit kidney, as well as in a non-excitable cell line, LLC-RK1, derived from rabbit kidney epithelial cells. Amplification by rapid amplification of 5' cDNA ends indicated the kidney type 2 ryanodine receptor transcript extended >7000 base pairs from the stop codon and is therefore not homologous to the short RyR-1 transcript of approximately 2500 base pairs previously observed in rabbit brain. [3H]Ryanodine binding and immunoblot analysis with a type 2 ryanodine receptor-specific antibody demonstrated that the native type 2 ryanodine receptor protein is expressed in the kidney. These observations suggest that the type 2 ryanodine receptor isoform may play a functional role in regulating intracellular calcium homeostasis in non-...Continue Reading

References

Jan 1, 1992·Annual Review of Physiology·C D Ferris, S H Snyder
Jan 1, 1992·Annual Review of Biochemistry·P W Majerus
Dec 1, 1992·The Biochemical Journal·F A LaiG Meissner
Jan 27, 1992·FEBS Letters·M S IslamP O Berggren
Aug 1, 1991·Experimental Cell Research·C MacDonaldR K Kinne
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Apr 1, 1989·Journal of Bioenergetics and Biomembranes·F A Lai, G Meissner
Jan 28, 1993·Nature·M J Berridge
Apr 1, 1993·The Biochemical Journal·A Sanchez-Bueno, P H Cobbold
Mar 1, 1993·Trends in Pharmacological Sciences·K Mikoshiba
Mar 1, 1993·Trends in Pharmacological Sciences·V Sorrentino, P Volpe
Apr 15, 1993·The Biochemical Journal·A H GuseF Emmrich

❮ Previous
Next ❯

Citations

Oct 12, 2000·Journal of Cellular Physiology·H Y KangW H Kang
Oct 24, 2003·Cell Calcium·Jason I E BruceDavid I Yule
Nov 17, 2007·Acta Physiologica·G Prinz, M Diener
Jan 27, 2012·Clinical and Experimental Nephrology·Takashi UdagawaTatsuo Hosoya
Jan 6, 2009·Annals of the New York Academy of Sciences·Dana JurkovicovaOlga Krizanova
Sep 16, 2006·The Journal of Biological Chemistry·Nicola PierobonAndrew P Thomas
Jan 18, 2008·American Journal of Physiology. Renal Physiology·Joseph SatrianoRoland C Blantz
Jun 16, 2001·American Journal of Physiology. Renal Physiology·A N YusufiJ P Grande
Apr 8, 1998·The American Journal of Physiology·C L ChouM A Knepper
Jun 27, 2019·Proceedings of the National Academy of Sciences of the United States of America·Sun-Ji ParkYing Maggie Chen
Jun 4, 1998·Biochemical and Biophysical Research Communications·P G Wood, J I Gillespie

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.