Ryanodine reveals multiple contractile and relaxant mechanisms in vascular smooth muscle: simultaneous measurements of mechanical activity and of cytoplasmic free Ca2+ level with fura-2

British Journal of Pharmacology
T HisayamaY Okamoto

Abstract

1. The effects of ryanodine on changes in cytoplasmic Ca2+ level ([Ca2+]i) and muscle tension induced by maximum concentrations of phenylephrine (Phe; 1 microM), prostaglandin F2 alpha (PGF2 alpha, 10 microM), caffeine (Caf, 30 mM) and isoprenaline (Iso, 1 microM) were examined in rat aortic strips using fura-2. 2. In normal media, Phe and PGF2 alpha produced a phasic contraction, followed by a tonic one. Caf elicited only a transient contraction. When the preparation was treated with 10 microM ryanodine, an increase in [Ca2+]i was induced accompanied by a nicardipine (1 microM)-resistant contraction which was [Ca2+]o-dependent. 3. In Ca2(+)-free solution, the three stimulants elicited transient increases in [Ca2+]i. Transient contractions to Phe and Caf were accompanied by changes in [Ca2+]i. The transient increase in [Ca2+]i induced by PGF2 alpha was not accompanied by a corresponding contraction. 4. Sustained contractions were induced by Phe and PGF2 alpha in the absence of external Ca2+, while the increase in [Ca2+]i was reduced. A larger maximum contraction was induced by PGF alpha than by Phe. 5. Ryanodine abolished both the Caf- and Phe-induced [Ca2+]i transient increases and the corresponding contractions, but had no su...Continue Reading

References

Jul 18, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·D F Fitzpatrick, A Szentivanyi
Sep 1, 1987·The American Journal of Physiology·E RousseauG Meissner
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L HymelH Schindler
Apr 15, 1988·Biochemical and Biophysical Research Communications·L HymelS Fleischer
Oct 14, 1988·Biochemical and Biophysical Research Communications·V Pijuan, I Litosch
Jan 1, 1985·Annual Review of Pharmacology and Toxicology·K E Kamm, J T Stull
Apr 29, 1987·European Journal of Pharmacology·E A Suba, B L Roth
Sep 1, 1988·Pflügers Archiv : European journal of physiology·T Hisayama, I Takayanagi
Dec 1, 1988·Pflügers Archiv : European journal of physiology·Y KanmuraR Casteels
Oct 1, 1988·British Journal of Pharmacology·G Julou-Schaeffer, J L Freslon
Apr 15, 1988·Biochemical and Biophysical Research Communications·M IinoM Endo
Apr 1, 1987·Pflügers Archiv : European journal of physiology·K S Hwang, C van Breemen
Jul 16, 1985·Biochemical and Biophysical Research Communications·H Yamamoto, C van Breemen
Mar 1, 1972·The Journal of Cell Biology·C E DevineA P Somlyo
Jul 1, 1984·British Journal of Pharmacology·C MironneauJ P Savineau
Dec 1, 1981·Pflügers Archiv : European journal of physiology·R CasteelsJ Van Eldere
May 1, 1982·Canadian Journal of Physiology and Pharmacology·R J Heaslip, R G Rahwan

❮ Previous
Next ❯

Citations

Jun 5, 1992·European Journal of Pharmacology·H ShimamotoE E Daniel
Feb 23, 1993·European Journal of Pharmacology·F Pérez-VizcaínoJ Tamargo
Mar 11, 1994·European Journal of Pharmacology·M KojimaK Sato
Apr 16, 1998·European Journal of Pharmacology·M TosunR M Rapoport
Jun 1, 1993·British Journal of Pharmacology·M D IvorraP D'Ocon
Apr 1, 1999·Annual Review of Physiology·T B BoltonD V Gordienko
Dec 17, 2005·The Journal of Physiology·Vladimir A SnetkovPhilip I Aaronson
Nov 3, 2005·American Journal of Physiology. Renal Physiology·Ming-Guo Feng, L Gabriel Navar
Jan 21, 2010·Physiological Reviews·Susan Wray, Theodor Burdyga
Aug 1, 1991·The American Journal of Physiology·E SuematsuK G Morgan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.