RYBP is important for cardiac progenitor cell development and sarcomere formation

PloS One
Surya HenryMelinda K Pirity

Abstract

We have previously established that epigenetic regulator RING1 and YY1 binding protein (RYBP) is required for the contractility of embryonic stem (ES) cell derived cardiomyocytes (CMCs), suggesting its essential role in contractility. In order to investigate the underlying molecular events of this phenotype, we compared the transcriptomic profile of the wild type and Rybp null mutant ES cells and CMCs differentiated from these cell lines. We identified genes related to ion homeostasis, cell adhesion and sarcomeric organization affected in the Rybp null mutant CMCs, by using hierarchical gene clustering and Gene Ontology analysis. We have also demonstrated that the amount of RYBP is drastically reduced in the terminally differentiated wild type CMCs whilst it is broadly expressed in the early phase of differentiation when progenitors form. We also describe that RYBP is important for the proper expression of key cardiac transcription factors including Mesp1, Shh and Mef2c. These findings identify Rybp as a gene important for both early cardiac gene transcription and consequent sarcomere formation necessary for contractility. Since impairment of sarcomeric function and contractility plays a central role in reduced cardiac pump fun...Continue Reading

References

Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A NagyJ C Roder
Aug 2, 2005·Molecular and Cellular Biology·Melinda K PirityNicole Schreiber-Agus
May 2, 2007·BMC Developmental Biology·Melinda K PirityAles Cvekl
Dec 11, 2008·Journal of Visualized Experiments : JoVE·Xiang Wang, Phillip Yang
Oct 8, 2009·Circulation. Arrhythmia and Electrophysiology·Augustus O Grant
May 4, 2010·Developmental Cell·Anthony A LanahanMichael Simons
Jun 16, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Fuhua ChenJames N Weiss
Mar 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Aibin HeWilliam T Pu
May 25, 2011·Cardiovascular Research·Jan Hendrick van WeerdJun K Takeuchi
Dec 14, 2011·Circulation Research·Aibin HeWilliam T Pu
Mar 1, 2012·Differentiation; Research in Biological Diversity·Faizal Z Asumda, P Bryant Chase
Mar 28, 2012·Current Topics in Developmental Biology·David J McCulley, Brian L Black
Feb 16, 2013·Circulation Research·Akl C FahedChristine E Seidman
May 28, 2013·Journal of the American Heart Association·Yoshihiro KuwabaraKazuwa Nakao
Oct 3, 2013·Cold Spring Harbor Perspectives in Medicine·Thomas BradeKarl-Ludwig Laugwitz
Oct 3, 2014·Cold Spring Harbor Perspectives in Medicine·Robert G KellyAntoon F Moorman
Dec 19, 2014·Nature·Zhonghua GaoDanny Reinberg
Jun 26, 2015·Stem Cells and Development·Olga UjhellyMelinda Katalin Pirity
Dec 30, 2015·Molecular and Cellular Biology·Carmela CalésMiguel Vidal
Nov 12, 2016·Development·Yu-Huan ShihXiaolei Xu
Aug 6, 2017·Circulation·Roberto PapaitGianluigi Condorelli
Nov 17, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Alexey V KharitonovEugene V Sheval

❮ Previous
Next ❯

Methods Mentioned

BETA
ChIP-seq

Software Mentioned

ImageJ
XLSTAT

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.