S-100 immunoreactivity in rat brain glial cultures is associated with both astrocytes and oligodendrocytes

Journal of Neuroscience Research
C Richter-Landsberg, M Heinrich

Abstract

S-100 protein, a Ca(2+)-binding protein of the EF-hand type, is most abundant in the brain, and is involved in cell differentiation and the molecular mechanisms underlying cytoskeletal organization. We have investigated the immunocytochemical localization of S-100 protein in rat brain glial cultures prepared from the cerebral hemispheres of newborn rats. In mixed glial cultures, containing astrocytes type I and II and oligodendrocytes at various stages of differentiation, S-100 immunoreactivity was detected in all three cell types. Double immunofluorescence analysis revealed that in astrocytes, S-100 immunoreactivity was mainly colocalized with glial fibrillary acidic protein (GFAP), while in oligodendrocytes a close association with microtubular structures was observed. For immunoblot analysis, highly enriched oligodendrocytes and astrocytes were separately cultured for another week, and their extracts were analyzed by immunoblotting. The immunoblots of the cell extracts of both cell types showed a single S-100-immunoreactive polypeptide with an apparent molecular weight of approximately 12,000 daltons. Thus, the data presented here demonstrate that S-100 protein is not confined to astrocytes but occurs also in oligodendrocyte...Continue Reading

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Citations

Jul 15, 1996·Journal of Neuroscience Research·C Richter-Landsberg, M Heinrich
Feb 5, 2000·Journal of Neuroscience Research·C Richter-Landsberg
Apr 23, 2003·Journal of Chemical Neuroanatomy·Claudia CastagnaGian Carlo Panzica
Nov 14, 2008·Neuron Glia Biology·Ximena A Lanosa, Jorge A Colombo
Sep 13, 2013·Journal of Child and Adolescent Psychopharmacology·Nathalie BockTill Manzke
Dec 22, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Chiu-Ming WenChung-Shung Wang
Feb 1, 2003·The Journal of Comparative Neurology·Virginie VivesCatherine Legraverend
Oct 27, 2006·Journal of Neurochemistry·Akiko HasegawaKazuhiro Ikenaka
Oct 15, 1998·Experimental Cell Research·C Richter-Landsberg, U Vollgraf
May 18, 2016·Neuroscience and Biobehavioral Reviews·Keisuke KawataDianne Langford
Oct 27, 2020·Frontiers in Cellular Neuroscience·Gisela SantosAdelaide Fernandes

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