PMID: 1208211Oct 1, 1975Paper

S-adenosylhomocysteine inhibition of three purified tRNA methyltransferases from rat liver

Nucleic Acids Research
J M GlickP S Leboy

Abstract

Three tRNA methyltransferases from rat liver have been fractionated and purified greater than 100-fold. These enzymes have been examined for their sensitivity to inhibition by S-adenosylhomocysteine (SAH). The methyltransferase which forms m2-guanine in the region between the dihydrouridine loop and the acceptor stem of tRNA (m2-guanine methyltransferase I) is least sensitive to SAH inhibition, with a Ki of 8 muM. The enzyme responsible for forming m2-guanine between the dihydrouridine and anticodon loops (m2-guanine methyltransferase II) has a Ki of 0.3 muM, while m1-adenine methyltransferase shows intermediate sensitivity to SAH (Ki = 2.4 muM). All three methyltransferases have similar Km's for the S-adenosylmethionine substrate (1.5-2.0 muM). These results are consistent with the hypothesis that activity of individual tRNA methyltransferases may be controlled by enzyme systems which alter cellular SAH levels.

References

May 1, 1971·Analytical Biochemistry·F SalvatoreS K Shapiro
Jan 1, 1974·Advances in Enzyme Regulation·S J Kerr, J E Heady
Jul 27, 1970·Biochemical and Biophysical Research Communications·Y Kuchino, S Nishimura
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Nov 1, 1974·Nucleic Acids Research·J Kraus, M Staehelin

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Citations

Jan 1, 1978·Advances in Enzyme Regulation·K R Harrap
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N M Kredich, M S Hershfield
Sep 1, 1976·Nucleic Acids Research·L W LuK Randerath
Apr 20, 2005·Journal of Bacteriology·Kelly BabbBrian Stevenson
Aug 23, 1978·Biochimica Et Biophysica Acta·P S LeboyR T Borchardt
Feb 18, 2016·Methods : a Companion to Methods in Enzymology·Fabian Muttach, Andrea Rentmeister
Jan 1, 1976·Biochimie·F Nau
Nov 1, 1982·Archives of Biochemistry and Biophysics·G PaolellaF Salvatore
Oct 30, 1978·Biochemical and Biophysical Research Communications·H Jakubowski, A Guranowski
Feb 20, 2019·International Journal of Molecular Sciences·Ruben EsseRita Castro
Oct 2, 2020·The Journal of Nutrition·Francois BlachierAnne Blais
Mar 29, 1978·Biochimica Et Biophysica Acta·J M GlickP S Leboy
Jan 29, 1985·Biochimica Et Biophysica Acta·T SindhuphakI Svensson
Dec 20, 1982·Biochimica Et Biophysica Acta·H Jakubowski

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