S-Adenosylmethionine Synthetase Is Required for Cell Growth, Maintenance of G0 Phase, and Termination of Quiescence in Fission Yeast

IScience
Takeshi HayashiMitsuhiro Yanagida

Abstract

S-adenosylmethionine is an important compound, because it serves as the methyl donor in most methyl transfer reactions, including methylation of proteins, nucleic acids, and lipids. However, cellular defects in the genetic disruption of S-adenosylmethionine synthesis are not well understood. Here, we report the isolation and characterization of temperature-sensitive mutants of fission yeast S-adenosylmethionine synthetase (Sam1). Levels of S-adenosylmethionine and methylated histone H3 were greatly diminished in sam1 mutants. sam1 mutants stopped proliferating in vegetative culture and arrested specifically in G2 phase without cell elongation. Furthermore, sam1 mutants lost viability during nitrogen starvation-induced G0 phase quiescence. After release from the G0 state, sam1 mutants could neither increase in cell size nor re-initiate DNA replication in the rich medium. Sam1 is thus required for cell growth and proliferation, and maintenance of and exit from quiescence. sam1 mutants lead to broad cellular and drug response defects, as expected, since S. pombe contains more than 90 S-adenosylmethionine-dependent methyltransferases.

Citations

Jul 10, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Jinhua ZhangHong Zhang
Sep 27, 2019·Frontiers in Genetics·Magdalini SerefidouAxel Imhof
Aug 26, 2021·Ecology and Evolution·Zhimeng XuHongbin Liu
Oct 17, 2021·FEBS Open Bio·Bhanu Pratap Singh ChouhanPaola Laurino

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Methods Mentioned

BETA
PCR
affinity purification
fluorescence-activated cell sorting

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