S-allylcysteine suppresses ovarian cancer cell proliferation by DNA methylation through DNMT1

Journal of Ovarian Research
Ya-si XuNeng-ming Lin

Abstract

The anti-tumor effects of S-allylcysteine (SAC), a water-soluble garlic derivative, on human ovarian cancer cells have been previous studied in vitro and in vivo models but the precise epigenetic molecular mechanisms are still unclear. This study aimed to investigate the epigenetic mechanism of SAC. Human epithelial ovarian cancer cell line A2780 was selected. Cell proliferation and cell cycle was analyzed. DNA methylation, DNA methyltransferase (DNMT) activity, tumor suppressor gene expressions, as well as protein expression were analyzed. SAC could inhibit the proliferation of A2780 cells in dose- and time-dependent manners (the IC50 was 16.25 mmol/L and 5.25 mmol/L at 48 h and 72 h). Treatment of A2780 cells with SAC resulted in G1/S phase arrest. SAC treatment decreased global DNA methylation levels in A2780 cells in a dose-dependent manner. SAC decreased the levels of 5-methylcytosine, DNMT activity, messenger RNA (mRNA) and protein levels of DNMT1. Additionally, SAC treatment resulted in re-expression of the mRNA and proteins of silenced tumor suppressor gene CDKN1A accompany with reduced cell division control 2 expression. Our data indicated the potential therapeutic effects of SAC on the human ovarian carcinoma cell lin...Continue Reading

References

Jun 4, 2002·Nature Reviews. Genetics·Peter A Jones, Stephen B Baylin
Oct 7, 2003·Oncogene·Adrian M Senderowicz
May 12, 2009·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Stefania NobiliSergio Capaccioli
Jun 2, 2009·Critical Reviews in Food Science and Nutrition·Masood Sadiq ButtJavaid Iqbal
Jul 2, 2009·Nature Reviews. Drug Discovery·Silvia Lapenna, Antonio Giordano
Aug 13, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Monika E HegiRoger Stupp
Jul 24, 2010·The American Journal of Pathology·Ernst Lengyel
Mar 9, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Kuang-Chi LaiJing-Gung Chung
Jun 27, 2012·International Journal of Cancer. Journal International Du Cancer·Winnie Lai Ting KanGe Lin
Nov 9, 2016·Advances in Experimental Medicine and Biology·Jiameng Dan, Taiping Chen
Mar 4, 2017·International Journal of Cancer. Journal International Du Cancer·S B CoburnB Trabert
Apr 27, 2017·Cancer Biology & Medicine·Brett M ReidThomas A Sellers
Nov 16, 2017·Pathology, Research and Practice·Suet Kee LooKah Keng Wong

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Citations

Nov 22, 2019·Anti-cancer Agents in Medicinal Chemistry·Rumana AhmadArvind K Srivastava
Mar 10, 2020·Pain Research & Management : the Journal of the Canadian Pain Society = Journal De La Société Canadienne Pour Le Traitement De La Douleur·Kun LiuGuang Han
Apr 10, 2019·Anti-cancer Agents in Medicinal Chemistry·Saleh A AlmatroodiArshad H Rahmani
Oct 22, 2020·Critical Reviews in Food Science and Nutrition·Ankita KumariRajeev Kapila
Dec 11, 2020·Seminars in Cancer Biology·Danielle De GreefAnupam Bishayee
Jun 2, 2021·Critical Reviews in Food Science and Nutrition·Xiaoming LuLi Ningyang
Jul 27, 2021·Environmental Science and Pollution Research International·Nasreddine El OmariAbdelhakim Bouyahya
Aug 28, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Arijit MondalAnupam Bishayee

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Methods Mentioned

BETA
flow cytometry
enzyme-linked immunosorbent
Assay
PCR
X-ray

Software Mentioned

GraphPad Prism
GraphPad
SPSS

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