S-equol inhibits proliferation and promotes apoptosis of human breast cancer MCF-7 cells via regulating miR-10a-5p and PI3K/AKT pathway

Archives of Biochemistry and Biophysics
Jian ZhangXiaoli Ye

Abstract

S-equol is the exclusive enantiomeric form of the soy isoflavone metabolite produced by human intestinal bacterial flora, which has strong anti-cancer activity. Based on this, the purpose of this study was to investigate the anti-breast cancer mechanism of S-equol. We examined the effects of S-equol on proliferation and apoptosis of MCF-7 cells by cell counting kit-8 assay and flow cytometry. Screening for microRNAs and predicting their target genes using the starBase and Targetscan website, respectively. Protein expression was detected by Western blot. The microRNA level were quantified by real-time PCR. The results showed that S-equol inhibited the proliferation of breast cancer MCF-7 cells in a time- and dose-dependent manner and promoted apoptosis of MCF-7 cells. The expression of miR-10a-5p was significantly decreased in breast cancer tissues and breast cancer cell lines, and the expression of miR-10a-5p was negatively correlated with the proliferation of MCF-7 cells. Luciferase reporter experiments demonstrated that miR-10a-5p directly targets PIK3CA 3'UTR to function. It was further found that S-equol exerts an anti-breast cancer effect by up-regulating miR-10a-5p and inhibiting the PI3K/AKT pathway. Our study revealed t...Continue Reading

Citations

Aug 23, 2020·Biomedicines·Elisa Sanchez-MorateCristina Mas-Bargues
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rafidah HodNurul Huda Mohd Nor
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Ludmila KřížováTomáš Kašparovský
May 19, 2021·The Kaohsiung Journal of Medical Sciences·Duo ShenWei Zhao

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