S gene mutants occurrence among hepatitis B carriers in malaysia

Hepatitis Monthly
Jeyanthi SuppiahZainah Saat

Abstract

The S gene region of the hepatitis B virus (HBV) codes for surface antigen (HBs Ag) and is responsible for classification of HBV strains. The current study aimed to identify important mutations in the S gene in Hepatitis B virus (HBV) isolated from Malaysian HBV carriers. Isolated HBV DNAs were subjected for PCR amplification and sequencing of HBV full genome. A total of 76 HBV full genome and 17 partial genome sequences were obtained from the 93 sequenced sera samples Genotyping of the full genome sequences by HEPSEQ software revealed a distribution of 49.46%, 48.39% and 2.15% of genotypes C, B, and D, respectively; whereas phylogenetic and jumping profile Hidden Markov Model (jpHMM) analysis identified six (7.89%) recombinant B/C strains. The distribution of sub-genotypes were B2 (78.79%) and B3 (21.21%) for genotype B, sub genotype D2 (100%) for genotype D and sub genotype C1 (75.76%), C2 (15.15%), C3 (6.06%) and C5 (3.13%) for genotype C. Mutation analysis in the S gene demonstrated two significant mutations which were W182 stop codon and deletion at open reading frame (ORF) of pre-S1 with the frequency occurrence of 2.2% (2/93) and 5.4% (5/93), respectively. The two patients with W182 stop codon were both male, infected wi...Continue Reading

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Datasets Mentioned

BETA
GQ924603
KJ803752-KJ803827‏
MY241913
MY851636

Methods Mentioned

BETA
PCR
electrophoresis
antisense oligonucleotides

Software Mentioned

CLUSTAL Omega
Mega
HEPSEQ
Genotyper

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