S-layer fusion protein as a tool functionalizing emulsomes and CurcuEmulsomes for antibody binding and targeting

Colloids and Surfaces. B, Biointerfaces
Mehmet H UcisikUwe B Sleytr

Abstract

Selective targeting of tumor cells by nanoparticle-based drug delivery systems is highly desirable because it maximizes the drug concentration at the desired target while simultaneously protecting the surrounding healthy tissues. Here, we show a design for smart nanocarriers based on a biomimetic approach that utilizes the building principle of virus envelope structures. Emulsomes and CurcuEmulsomes comprising a tripalmitin solid core surrounded by phospholipid layers are modified by S-layer proteins that self-assemble into a two-dimensional array to form a surface layer. One significant advantage of this nanoformulation is that it increases the solubility of the lipophilic anti-cancer agent curcumin in the CurcuEmulsomes by a factor of 2700. In order to make the emulsomes specific for IgG, the S-layer protein is fused with two protein G domains. This S-layer fusion protein preserves its recrystallization characteristics, forming an ordered surface layer (square lattice with 13 nm unit-by-unit distance). The GG domains are presented in a predicted orientation and exhibit a selective binding affinity for IgG.

References

Jan 1, 1979·Journal of Immunological Methods·R C DuhamelE Meezan
Sep 1, 2000·Advanced Drug Delivery Reviews·S P Vyas, V Sihorkar
Dec 14, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fenghua MengJan Feijen
Feb 12, 2009·ACS Nano·Omid C Farokhzad, Robert Langer
Aug 3, 2011·Colloids and Surfaces. B, Biointerfaces·Neda HabibiCarmelina Ruggiero
Apr 23, 2013·Small·Mehmet H UcisikUwe B Sleytr
Feb 4, 2014·FEMS Microbiology Reviews·Uwe B SleytrDietmar Pum

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Citations

Apr 12, 2018·Biosensors·Bernhard Schuster
Feb 22, 2017·International Journal of Molecular Sciences·Andreas BreitwieserDietmar Pum

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Methods Mentioned

BETA
PCRs
PCR
Protein Extraction
ELISA
Light Scattering
AFM

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