S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization

Brazilian Oral Research
Renzo Alberto Ccahuana-Vásquez, Jaime Aparecido Cury

Abstract

The aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37 degrees C, 10% CO2. To validate the model, the biofilms were treated 2x/day with chlorhexidine digluconate (CHX) at 0.012, 0.024 and 0.12% (concentration with recognized anti-plaque effect) and 0.05% NaF (concentration with recognized anti-caries effect). CHX showed dose-response effect decreasing biomass, bacterial viability and enamel demineralization (p < 0.05). Whereas, 0.05% NaF did not show antimicrobial effect but had similar effect to that of 0.12% CHX decreasing enamel demineralization (p < 0.05). The model developed has potential to evaluate the effect of substances on biofilm growth and on enamel demineralization.

References

Jun 1, 1975·Journal of Dental Research·G Rölla, B Melsen
Apr 1, 1989·Scandinavian Journal of Dental Research·G Rölla
Apr 1, 1997·Advances in Dental Research·J Carlsson
Apr 1, 1997·Advances in Dental Research·C H Sissons
Jun 27, 1998·Periodontology 2000·C G Jones
Apr 25, 2000·Acta Odontologica Scandinavica·J M ten Cate
Feb 13, 2001·Journal of Microbiological Methods·B HerigstadJ Heersink
Oct 1, 1964·The Journal of Pharmacy and Pharmacology·W B HUGO, A R LONGWORTH
Aug 13, 2008·Advances in Dental Research·V C C Marinho
Aug 13, 2008·Advances in Dental Research·J A Cury, L M A Tenuta
Oct 4, 2008·Caries Research·N Takahashi, B Nyvad
Mar 17, 2009·Journal of Microbiological Methods·D M DengW Crielaard
Aug 10, 2010·International Journal of Oral Science·Hyun KooMarlise I Klein

❮ Previous
Next ❯

Citations

Oct 10, 2012·European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry·D N SoaresL C Maia
Dec 23, 2015·International Journal of Biological Macromolecules·Carolina P AiresJaime A Cury
Dec 17, 2015·Journal of Oral Science·Selcuk SavasMustafa Ates
Jun 21, 2014·BioMed Research International·Mariana P De LucaVagner R Santos
Jan 29, 2013·Acta Odontologica Scandinavica·Daniela Novaes SoaresLucianne Cople Maia
May 2, 2013·Archives of Oral Biology·Rodrigo A GiacamanRamiro J Castro
Apr 16, 2014·Biofouling·Yuri Wanderley CavalcantiJaime Aparecido Cury
Jun 14, 2016·Scientifica·Vanessa Salvadego de QueirozAngélica Zaninelli Schreiber
May 2, 2016·Journal of Dentistry·Christina P C SimEric C Reynolds
Jul 13, 2016·European Journal of Dentistry·Natalia Díaz-GarridoRodrigo A Giacaman
May 26, 2016·Brazilian Oral Research·Juliana Nunes BotelhoJaime Aparecido Cury
Jul 14, 2012·Brazilian Oral Research·Cecília Cláudia Costa RibeiroJaime Aparecido Cury
Jun 7, 2018·Caries Research·Manuela S SpinolaLivia Maria Andaló Tenuta
Feb 15, 2018·Caries Research·Samilly Evangelista SouzaJaime Aparecido Cury
Oct 1, 2017·Lasers in Medical Science·Ana Caroline FumesMaria Cristina Borsatto
May 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Erika R H KawakitaCarolina P Aires
Jul 18, 2017·PloS One·Bárbara Emanoele Costa OliveiraAntônio Pedro Ricomini Filho
Dec 14, 2018·Clinical Oral Investigations·Rodrigo A GiacamanCecilia Muñoz-Sandoval
Feb 28, 2019·Scientific Reports·Laryssa de Barros PintoFrederico Barbosa de Sousa
Aug 23, 2020·Applied and Environmental Microbiology·Natalia Díaz-GarridoRodrigo A Giacaman
Oct 30, 2016·Journal of Applied Biomaterials & Functional Materials·Ebru Kucukyilmaz, Selcuk Savas
Mar 17, 2020·Photodiagnosis and Photodynamic Therapy·Leonardo Lobo Ribeiro CavalcanteSilmara Aparecida Milori Corona
Mar 7, 2021·Microorganisms·María Carmen SánchezMariano Sanz
Jan 19, 2020·Dental Materials : Official Publication of the Academy of Dental Materials·Fernando Luis Esteban FlorezSharukh Soli Khajotia
May 10, 2019·Archives of Oral Biology·Naile Damé-TeixeiraThuy Do
Aug 6, 2019·Archives of Oral Biology·Mariana Leonel MartinsAndréa Fonseca-Gonçalves
May 15, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Edilson Martins Rodrigues NetoMarta Maria de França Fonteles

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.