S-Nitrosylation in Organs of Mice Exposed to Low or High Doses of γ-Rays: The Modulating Effect of Iodine Contrast Agent at a Low Radiation Dose

Proteomes
Fadia NicolasEdouard I Azzam

Abstract

The covalent addition of nitric oxide (NO(•)) onto cysteine thiols, or S-nitrosylation, modulates the activity of key signaling proteins. The dysregulation of normal S-nitrosylation contributes to degenerative conditions and to cancer. To gain insight into the biochemical changes induced by low-dose ionizing radiation, we determined global S-nitrosylation by the "biotin switch" assay coupled with mass spectrometry analyses in organs of C57BL/6J mice exposed to acute 0.1 Gy of (137)Cs γ-rays. The dose of radiation was delivered to the whole body in the presence or absence of iopamidol, an iodinated contrast agent used during radiological examinations. To investigate whether similar or distinct nitrosylation patterns are induced following high-dose irradiation, mice were exposed in parallel to acute 4 Gy of (137)Cs γ rays. Analysis of modulated S-nitrosothiols (SNO-proteins) in freshly-harvested organs of animals sacrificed 13 days after irradiation revealed radiation dose- and contrast agent-dependent changes. The major results were as follows: (i) iopamidol alone had significant effects on S-nitrosylation in brain, lung and liver; (ii) relative to the control, exposure to 0.1 Gy without iopamidol resulted in statistically-signi...Continue Reading

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Citations

Mar 28, 2017·Mutation Research. Reviews in Mutation Research·Janet HallElisabeth Cardis
Jul 18, 2019·Molecular & Cellular Proteomics : MCP·Monika Zareba-KoziolJakub Wlodarczyk
Jun 20, 2019·Journal of Radiological Protection : Official Journal of the Society for Radiological Protection·Edouard I Azzam
Oct 1, 2019·Molecular & Cellular Proteomics : MCP·Monika Zareba-KoziolJakub Wlodarczyk

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Methods Mentioned

BETA
X-ray
electrophoresis
immunoprecipitation

Software Mentioned

Ingenuity Pathway Analysis ( IPA )
PANTHER
Excel
ImageJ
Mascot
Ingenuity
ProteinLynx
PANTHER ( Protein Analysis Through Evolutionary Relationships )
IPA
Ingenuity Pathway Analysis ( IPA

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