S. pombe kinesins-8 promote both nucleation and catastrophe of microtubules.

PloS One
Muriel ErentRobert A Cross

Abstract

The kinesins-8 were originally thought to be microtubule depolymerases, but are now emerging as more versatile catalysts of microtubule dynamics. We show here that S. pombe Klp5-436 and Klp6-440 are non-processive plus-end-directed motors whose in vitro velocities on S. pombe microtubules at 7 and 23 nm s(-1) are too slow to keep pace with the growing tips of dynamic interphase microtubules in living S. pombe. In vitro, Klp5 and 6 dimers exhibit a hitherto-undescribed combination of strong enhancement of microtubule nucleation with no effect on growth rate or catastrophe frequency. By contrast in vivo, both Klp5 and Klp6 promote microtubule catastrophe at cell ends whilst Klp6 also increases the number of interphase microtubule arrays (IMAs). Our data support a model in which Klp5/6 bind tightly to free tubulin heterodimers, strongly promoting the nucleation of new microtubules, and then continue to land as a tubulin-motor complex on the tips of growing microtubules, with the motors then dissociating after a few seconds residence on the lattice. In vivo, we predict that only at cell ends, when growing microtubule tips become lodged and their growth slows down, will Klp5/6 motor activity succeed in tracking growing microtubule t...Continue Reading

References

Nov 9, 1989·Nature·J HowardR D Vale
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Nov 15, 1984·Nature·T Mitchison, M Kirschner
May 1, 1995·Molecular Biology of the Cell·K M Huang, M D Snider
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A J Hunt, J Howard
Nov 21, 1997·Journal of Molecular Biology·I M CrevelR A Cross
Nov 13, 1998·The Journal of Cell Biology·A F StraightA W Murray
Jul 19, 2000·Current Biology : CB·D R Drummond, R A Cross
Apr 20, 2001·The Journal of Cell Biology·P T TranF Chang
Sep 19, 2002·The Journal of Biological Chemistry·Cori N NewtonLeslie Wilson
Jun 25, 2003·The Journal of Cell Biology·Marcel E JansonMarileen Dogterom
Sep 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michael P YaffeRonald D Vale
Sep 19, 2003·Molecular Biology of the Cell·Rita GandhiAndrea Pereira
Nov 20, 2003·Journal of Cell Science·Meredith Johnson SagollaW Zacheus Cande
Mar 6, 2004·Science·Henrik BringmannThomas Surrey
May 20, 2005·Nature·N J Carter, R A Cross
Aug 5, 2005·The EMBO Journal·Isabel Sanchez-PerezJonathan B A Millar
Aug 9, 2005·Current Biology : CB·Lisa R SproulSusan P Gilbert
Dec 13, 2005·Nature Reviews. Molecular Cell Biology·Tomoyuki U TanakaKozo Tanaka
Dec 14, 2005·Biochemical and Biophysical Research Communications·Takuya KobayashiYoko Y Toyoshima
Feb 17, 2006·Molecular Biology of the Cell·Bill Wickstead, Keith Gull
Aug 15, 2006·Nature Cell Biology·Vladimir VargaJonathon Howard
Mar 6, 2007·Developmental Cell·Johanna L HöögClaude Antony
Apr 7, 2007·Science·Maria C AlonsoRobert A Cross
Jan 30, 2008·Current Protocols in Cell Biology·C M Waterman-Storer

❮ Previous
Next ❯

Citations

Mar 13, 2014·The Journal of Cell Biology·Rania S RizkMohan L Gupta
Aug 20, 2014·Systems and Synthetic Biology·Liam J Messin, Jonathan B A Millar
Sep 12, 2014·Molecular Biology of the Cell·Haein KimJason Stumpff
Jul 24, 2013·Annual Review of Cell and Developmental Biology·Claire E WalczakRyoma Ohi
Jul 1, 2015·The Journal of Cell Biology·Damien LaporteIsabelle Sagot
Feb 13, 2013·The Journal of Cell Biology·Masashi YoshidaAyumu Yamamoto
Feb 13, 2016·Scientific Reports·Frauke HussmannRobert A Cross
Sep 11, 2012·Trends in Cell Biology·Xiaolei SuDavid Pellman
Feb 25, 2015·Experimental Cell Research·Juan Jesus Vicente, Linda Wordeman
May 6, 2016·Annual Review of Biophysics·Nenad Pavin, Iva M Tolić
Sep 16, 2016·Scientific Reports·Daniele CartelliGraziella Cappelletti
Nov 5, 2016·Molecular Biology of the Cell·Zachary R GergelyMeredith D Betterton
Sep 21, 2012·Molecular Biology of the Cell·Per O WidlundDavid N Drechsel
Oct 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Julia LockeCarolyn A Moores
Apr 17, 2013·Physical Biology·Hui-Shun Kuan, M D Betterton
Mar 13, 2015·Physical Review Letters·Matko GlunčićIva M Tolić
Dec 13, 2018·The Journal of Cell Biology·Tomoya Edzuka, Gohta Goshima
Apr 11, 2020·Biomolecules·Michael P Koonce, Irina Tikhonenko
Nov 22, 2013·Biochemical Society Transactions·John C Meadows
Oct 25, 2012·Current Opinion in Cell Biology·Melissa K GardnerJonathon Howard
Aug 5, 2021·Journal of Cell Science·Alberto Pineda-SantaellaAlfonso Fernández-Álvarez

❮ Previous
Next ❯

Methods Mentioned

BETA
affinity purification
Contrast
light scattering
gel filtration
PCR
motility
motility assay
fluorescence microscopy

Software Mentioned

RETRAC
GraphPad
VE
Prism
Kaleidagraph
Synergy
MetaMorph
Quantity one
NIH
DIC

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.