S-thiolation of HSP27 regulates its multimeric aggregate size independently of phosphorylation.

The Journal of Biological Chemistry
Philip EatonMichael J Shattock

Abstract

HSP27 exists as large aggregates that breakdown after phosphorylation. We show rat cardiac HSP27 is S-thiolated during oxidant stress, and this modification, without phosphorylation, disaggregates multimeric HSP27. Biotinylated cysteine acts as a probe for thiolated proteins, which are detected using non-reducing Western blots probed with streptavidin-horseradish peroxidase. Controls show a low level of S-thiolation, which is increased 3.6-fold during post-ischemic reperfusion. S-thiolated proteins were purified using streptavidin-agarose, and Western immunoblotting showed HSP27 was present. We increased protein S-thiolation 10-fold with 10 microm H2O2 with or without a kinase inhibitor mixture (staurosporine, genistein, bisindolylmaleimide, SB203580, and PD98059). H2O2 alone induced the phosphorylation of HSP27 Ser-86 and Ser-45/Ser-59 of its homologue alphaB crystallin. However, kinase inhibition reduced phosphorylation of these sites below basal. Despite effective kinase inhibition, H2O2 still disaggregated HSP27, but not alphaB crystallin. This is consistent with the lack of an S-thiolation site on alphaB crystallin. Thus, we have demonstrated a novel mechanism of HSP27 multimeric size regulation. S-thiolation must occur at...Continue Reading

References

Aug 1, 1992·Circulation Research·F BennardiniM Chiesi
Oct 1, 1991·Cardiovascular Drugs and Therapy·D J Hearse
Apr 22, 1987·Biochimica Et Biophysica Acta·M W Collison, J A Thomas
Aug 1, 1993·Cardiovascular Research·C Ganote, S Armstrong
Dec 6, 1995·Biochimica Et Biophysica Acta·I V DudichT Korpela
May 1, 1996·Circulation Research·R BarbatoF Di Lisa
Jul 1, 1996·Current Eye Research·C QinJ S Zigler
Dec 31, 1997·The Journal of Biological Chemistry·H ItoK Kato
Feb 19, 1998·Archives of Biochemistry and Biophysics·P Y CheungR Schulz
Jun 20, 1998·The Journal of Biological Chemistry·N E WardC A O'Brian
Jul 3, 1998·International Journal of Biological Macromolecules·D W Hook, J J Harding
Jul 11, 1998·The Journal of Biological Chemistry·T L Vanden HoekP T Schumacker
Aug 1, 1998·Circulation Research·I J Benjamin, D R McMillan
Jan 26, 1999·Archives of Biochemistry and Biophysics·Y JiJ A Thomas
Feb 20, 1999·Cell·U JakobJ C Bardwell
Apr 27, 1999·Progress in Retinal and Eye Research·B K Derham, J J Harding
Aug 5, 2000·Free Radical Biology & Medicine·R Gopalakrishna, S Jaken
Mar 21, 2001·The Biochemical Journal·R J MallisJ A Thomas
May 8, 2001·Archives of Biochemistry and Biophysics·M DemasiK J Davies
Oct 31, 2001·The Journal of Biological Chemistry·J WangP B Chock

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Citations

Nov 11, 2003·Free Radical Biology & Medicine·Isabella Dalle-DonneAldo Milzani
Jan 24, 2006·Preparative Biochemistry & Biotechnology·Kyoung-Soo ChoiJong Hoon Park
Jul 7, 2005·Antioxidants & Redox Signaling·Philip EatonMichael J Shattock
Dec 21, 2007·Antioxidants & Redox Signaling·Isabella Dalle-DonneRanieri Rossi
Apr 29, 2015·Expert Review of Proteomics·Evgeny V Mymrikov, Martin Haslbeck
May 10, 2008·Experimental Lung Research·Robert M Jackson, Rolando Garcia-Rojas
Mar 1, 2011·International Journal of Radiation Oncology, Biology, Physics·Seo-Hyun ChoiYun-Sil Lee
Jul 4, 2003·The Journal of Investigative Dermatology·Chun-Ming HuangDe-chu C Tang
Mar 21, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Evaldo ReischlDanilo Wilhelm Filho
Nov 25, 2006·Experimental Cell Research·A L BryantsevE A Shelden
Jul 13, 2004·The European Journal of Neuroscience·Arjen MaatkampDick Jaarsma
Jul 23, 2005·IUBMB Life·Isabella Dalle-DonneAldo Milzani
Jul 17, 2004·Journal of Cellular and Molecular Medicine·Daniela GiustariniIsabella Dalle-Donne
Mar 13, 2010·Journal of Cellular Biochemistry·Nagarekha PasupuletiRam H Nagaraj
Jun 24, 2004·Biochimica Et Biophysica Acta·Zhijun QiuJames S Clegg
Jan 31, 2006·Free Radical Biology & Medicine·Aimee LandarVictor M Darley-Usmar
Oct 15, 2005·Molecular & Cellular Proteomics : MCP·Jonathan P BrennanPhilip Eaton
Jun 16, 2007·Molecular & Cellular Proteomics : MCP·Rebecca L CharlesPhilip Eaton
Dec 1, 2017·Cell Stress & Chaperones·Ivan DimauroDaniela Caporossi
Aug 22, 2002·The Journal of Biological Chemistry·Kenneth M HumphriesSusan S Taylor
Sep 25, 2004·Mass Spectrometry Reviews·Isabella Dalle-DonneAldo Milzani
Nov 8, 2006·Photosynthesis Research·Laure MicheletStéphane D Lemaire
Jun 1, 2008·Proteomics. Clinical Applications·Rebecca L Charles, Philip Eaton
Jun 1, 2008·Proteomics. Clinical Applications·Axel GödeckeMichael Reinartz
Apr 26, 2005·Journal of Biochemical and Molecular Toxicology·Smarajit MaitiGuangping Chen
Oct 21, 2011·Physiological Reviews·Evgeny V MymrikovNikolai B Gusev
Feb 10, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Marcella CantonFabio Di Lisa
Feb 26, 2020·International Journal of Molecular Sciences·Jaakko SarparantaBjarne Udd
May 20, 2006·Molecular and Cellular Biochemistry·Catherine GaitanakiIsidoros Beis
Jan 12, 2017·Cell Stress & Chaperones·Nikolaos CharmpilasNektarios Tavernarakis
Jul 6, 2012·American Journal of Physiology. Cell Physiology·Noni T LarkinsGraham D Lamb
Oct 18, 2019·Frontiers in Pharmacology·Jack M WebsterLaura J Blair
Apr 5, 2018·Frontiers in Physiology·Claudia PennaMara Brancaccio
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Philip EatonMichael J Shattock

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