S1 ribosomal protein functions in translation initiation and ribonuclease RegB activation are mediated by similar RNA-protein interactions: an NMR and SAXS analysis

The Journal of Biological Chemistry
Pascale AliprandiFrancois Bontems

Abstract

The ribosomal protein S1, in Escherichia coli, is necessary for the recognition by the ribosome of the translation initiation codon of most messenger RNAs. It also participates in other functions. In particular, it stimulates the T4 endoribonuclease RegB, which inactivates some of the phage mRNAs, when their translation is no longer required, by cleaving them in the middle of their Shine-Dalgarno sequence. In each function, S1 seems to target very different RNAs, which led to the hypothesis that it possesses different RNA-binding sites. We previously demonstrated that the ability of S1 to activate RegB is carried by a fragment of the protein formed of three consecutive domains (domains D3, D4, and D5). The same fragment plays a central role in all other functions. We analyzed its structural organization and its interactions with three RNAs: two RegB substrates and a translation initiation region. We show that these three RNAs bind the same area of the protein through a set of systematic (common to the three RNAs) and specific (RNA-dependent) interactions. We also show that, in the absence of RNA, the D4 and D5 domains are associated, whereas the D3 and D4 domains are in equilibrium between open (noninteracting) and closed (weak...Continue Reading

References

Jan 11, 1991·Nucleic Acids Research·I V BoniN V Tzareva
Mar 1, 1990·Trends in Genetics : TIG·J E McCarthy, C Gualerzi
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R A CalogeroC O Gualerzi
Jan 1, 1983·Progress in Nucleic Acid Research and Molecular Biology·A R Subramanian
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Sep 1, 1995·Journal of Biomolecular NMR·D S WishartB D Sykes
Jan 1, 1997·Nucleic Acids Research·T J HubbardC Chothia
Dec 24, 1998·Nucleic Acids Research·D E Draper, L P Reynaldo
Feb 3, 1999·Annual Review of Genetics·M SugiuraM Sugita
Oct 3, 2000·Nature·B T WimberlyV Ramakrishnan
Jul 20, 2001·Methods in Enzymology·D S Wishart, D A Case
Aug 9, 2001·Journal of Molecular Biology·K SnoussiJ L Leroy
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J SenguptaJ Frank
Jul 16, 2003·The Journal of Biological Chemistry·Olga M SelivanovaAlexander S Spirin
Sep 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kathleen E McGinness, Robert T Sauer
Nov 8, 2005·Science·Barbara S SchuwirthJamie H Doudna Cate
Jun 16, 2006·RNA·Maxim V SukhodoletsSankar Adhya
Dec 8, 2006·FEBS Letters·Sultan Ch AgalarovAlexander S Spirin
Sep 29, 2007·RNA·Lukas Rajkowitsch, Renée Schroeder

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Citations

Aug 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xiaohui QuIgnacio Tinoco
Jul 17, 2009·Nucleic Acids Research·Philippe SalahFrançois Bontems
Mar 8, 2012·Molekuliarnaia biologiia·L V Aseev, I V Boni
Apr 20, 2010·Current Colorectal Cancer Reports·Shrikant AnantSatish Ramalingam
Mar 1, 2012·Biochimie·Eliane Hajnsdorf, Irina V Boni
Jan 11, 2011·Biochemical and Biophysical Research Communications·P MacheboeufP Vachette
Nov 19, 2015·Nucleic Acids Research·Heidi GytzCharlotte R Knudsen
Oct 13, 2015·BioMed Research International·Kai-Chun ChangLee-Wei Yang
Apr 1, 2014·Biomolecular NMR Assignments·Pierre GiraudChristina Sizun
Dec 17, 2014·Nucleic Acids Research·Konstantin ByrgazovIsabella Moll
Dec 14, 2019·Nucleic Acids Research·Paul E LundNils G Walter
Jul 2, 2020·International Journal of Molecular Sciences·Kwanuk Lee, Hunseung Kang

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