S100A11 protects against neuronal cell apoptosis induced by cerebral ischemia via inhibiting the nuclear translocation of annexin A1

Cell Death & Disease
Qian XiaJing Shi

Abstract

The subcellular location of annexin A1 (ANXA1) determines the ultimate fate of neurons after ischemic stroke. ANXA1 nuclear translocation is involved in neuronal apoptosis after cerebral ischemia, and extracellular ANXA1 is also associated with regulation of inflammatory responses. As the factors and mechanism that influence ANXA1 subcellular translocation remain unclear, studies aiming to determine and clarify the role of ANXA1 as a cell fate 'regulator' within cells are critically needed. In this study, we found that intracerebroventricular injection of the recombinant adenovirus vector Ad-S100A11 (carrying S100A11) strongly improved cognitive function and induced robust neuroprotective effects after ischemic stroke in vivo. Furthermore, upregulation of S100A11 protected against neuronal apoptosis induced by oxygen-glucose deprivation and reoxygenation (OGD/R) in vitro. Surprisingly, S100A11 overexpression markedly decreased ANXA1 nuclear translocation and subsequently alleviated OGD/R-induced neuronal apoptosis. Notably, S100A11 exerted its neuroprotective effect by directly binding ANXA1. Importantly, S100A11 directly interacted with ANXA1 through the nuclear translocation signal (NTS) of ANXA1, which is essential for ANXA1...Continue Reading

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Citations

Sep 24, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Arseniy E YuzhalinRuth J Muschel
Mar 4, 2021·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Junna HeRong Chen
Mar 6, 2021·Molecular Therapy. Methods & Clinical Development·Jing LuoYin Zhao
Apr 4, 2021·International Journal of Molecular Sciences·Thomas GrewalUrsula Rescher
Mar 24, 2021·Cardiovascular Research·Changping ZhouLemin Zheng
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Linqiang ZhangBin Liang

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Methods Mentioned

BETA
nuclear translocation
Co-IP
PCR
confocal microscopy
FRET
Fluorescence resonance
Fluorescence
transfection
flow cytometry
dissection

Software Mentioned

ImageJ

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