S100A6 is transcriptionally regulated by β-catenin and interacts with a novel target, lamin A/C, in colorectal cancer cells

Cell Calcium
Ewa KilańczykAnna Filipek

Abstract

In this paper we document an increased expression of S100A6, a calcium binding protein of the S100 family, and its co-localization with β-catenin in colorectal cancer tissues and in metastatic, SW620, versus non-metastatic, SW480, human colorectal cancer cell lines. Moreover, we show up-regulation of the S100A6 protein level in non-metastatic SW480 cells due to overexpression of β-catenin as well as the activation of the S100A6 gene promoter upon cell transfection with β-catenin and the TCF-Lef1 transcription factor. Since we found a high level of S100A6 in metastatic SW620 cells we searched for its interacting partners in the protein extract prepared from these cells. Using several methods we found that S100A6 interacts with lamin A/C, a protein known to be implicated in colon carcinogenesis. Our results reveal a novel and important network of relations and interactions between proteins potentially involved in colorectal cancer development and progression.

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Citations

Apr 30, 2013·Journal of Dermatological Science·Li ZhuMasutaka Furue
Jul 3, 2013·Neurochemical Research·Ewelina JurewiczAnna Filipek
Feb 18, 2016·Gastroenterology Research and Practice·Paula MoravkovaJan Bures
Jun 21, 2013·Journal of Proteome Research·Juan Martínez-Aguilar, Mark P Molloy
Jul 9, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Carolin LerchenmüllerPatrick Most
Jun 6, 2015·The Journal of Obstetrics and Gynaecology Research·Zequn LiuDonghua Huang
Apr 18, 2020·PloS One·Eva Nora BukoszaChristoph A Gebeshuber
Mar 28, 2017·Biological Chemistry·Wiesława LeśniakAnna Filipek
Jun 5, 2020·International Journal of Molecular Sciences·Anna Filipek, Wiesława Leśniak
Dec 21, 2019·Scandinavian Journal of Clinical and Laboratory Investigation·Paula MoravkovaJan Bures
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eva PeterovaDarina Kohoutova
Aug 7, 2021·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Ruicen LiHuairong Tang

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