S100A9 Increases IL-6 and RANKL Expressions through MAPKs and STAT3 Signaling Pathways in Osteocyte-Like Cells

BioMed Research International
Ryosuke TakagiHiromichi Yumoto

Abstract

Calprotectin is a heterocomplex of S100A8 and S100A9 and is mainly secreted from neutrophils, monocytes, and chondrocytes in inflammatory condition. Calprotectin binds to RAGE and TLR4 and induces the expression of proinflammatory chemokines and cytokines in various cells. Periodontitis is a chronic inflammatory disease that leads to gingival inflammation and alveolar bone resorption. Calprotectin levels in gingival crevicular fluid of periodontitis patients are higher than healthy patients. In the present study, the effects of S100A8 and S100A9 on the expressions of proinflammatory cytokines and bone metabolism-related factors in mouse osteocyte-like cells (MLO-Y4-A2) were investigated. MLO-Y4-A2 cells were treated with S100A8 and S100A9, and the expressions of RAGE, TLR4, RANKL, and several inflammatory cytokines were analyzed by PCR and Western blotting or ELISA methods. To investigate the intracellular signaling pathways, phosphorylation of MAPK and STAT3 was determined by Western blotting, and chemical specific inhibitors and siRNAs were used. Expressions of IL-6 and RANKL were increased by treatment with S100A9 but not S100A8. However, both S100A8 and S100A9 did not change expression of IL-1β, IL-8, and TNF-α. Although RA...Continue Reading

References

Jul 1, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Nanjun Wu, Jeffrey M Davidson
Nov 9, 2006·Experimental Lung Research·Markus O HenkeJa Seok Koo
Jul 20, 2007·Journal of Molecular Histology·H ZreiqatC L Geczy
Mar 6, 2010·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·G Y RochefortC L Benhamou
Mar 10, 2010·Amino Acids·Jesse Goyette, Carolyn L Geczy
Apr 25, 2012·Current Osteoporosis Reports·Tobias Braun, Georg Schett
Mar 28, 2013·International Journal of Dentistry·N SuzukiT Hirofuji
Sep 5, 2013·Calcified Tissue International·Teresita Bellido
Oct 15, 2013·Journal of Bone and Mineral Metabolism·Shoichi KaneshiroJun Hashimoto
Jan 22, 2014·Journal of Cellular Physiology·Kyunghwa BaekJeong-Hwa Baek
Apr 11, 2014·BioMed Research International·Bin ChenYin Xiao
Feb 28, 2015·Gerontology·Vanja Nagy, Josef M Penninger
Jun 10, 2015·The Journal of Biological Chemistry·Joseph P ZackularEric P Skaar
Jul 25, 2015·Journal of Tissue Engineering and Regenerative Medicine·Bin MaCharles James Kirkpatrick
Dec 23, 2015·Current Molecular Biology Reports·Jesus Delgado-Calle, Teresita Bellido
Aug 6, 2016·Pharmacology & Therapeutics·Monika PruensterMarkus Sperandio
Dec 8, 2016·Journal of Cellular Physiology·Yasufumi NishikawaKoji Naruishi
Mar 10, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Qing WuFeiwu Kang
Jun 8, 2017·Frontiers in Cellular and Infection Microbiology·Maja Sochalska, Jan Potempa
Jul 29, 2017·Journal of Periodontology·Ronaldo Lira-JuniorElisabeth A Boström
Jun 6, 2018·Frontiers in Physiology·Ana Alonso-PérezRodolfo Gómez
Jul 10, 2018·Rheumatology·Georg Schett
Aug 8, 2018·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Fatemeh ShabaniNematollah Gheibi
Oct 28, 2018·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Xiaonan ChenFang Wu
Nov 6, 2018·Disease-a-month : DM·R Hegde, K H Awan
Feb 20, 2019·Frontiers in Immunology·Marion MussbacherJohannes A Schmid

❮ Previous
Next ❯

Citations

Dec 22, 2020·Frontiers in Pharmacology·Larissa SteigmannWilliam V Giannobile
Mar 7, 2021·Journal of Ethnopharmacology·Paula Schons VieceliCristiane Flora Villarreal

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
protein assay
electrophoresis
transfection
ELISA
transfections

Software Mentioned

Image Quant
Stat view
NIH image

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.