S4(13)-PV cell penetrating peptide and cationic liposomes act synergistically to mediate intracellular delivery of plasmid DNA

The Journal of Gene Medicine
Sara TrabuloMaria C Pedroso de Lima

Abstract

Cell penetrating peptides have been successfully used to mediate the intracellular delivery of a wide variety of molecules of pharmacological interest. The main aim of the present work was to evaluate the potential of the S4(13)-PV cell penetrating peptide to mediate the intracellular delivery of plasmid DNA, aiming at its use in gene therapy applications. The S4(13)-PV cell penetrating peptide is a chimeric peptide that results from the combination of a cell penetrating sequence derived from the Dermaseptin S4 peptide with the nuclear localization signal present in the Simian Virus 40 (SV40) large T antigen. S4(13)-PV cell penetrating peptide and cationic liposomes composed of 1,2-dioleoyl-3-trimethylammonium-propane:1,2-dioleoyl-sn-glycero-3-phosphoethanolamine were complexed with pDNA at different charge ratios. Complexation of pDNA was assessed by gel electrophoresis. Luciferase assay, fluorescence microscopy and fluorescence-activated cell sorting analysis were used to evaluate reporter gene delivery to TSA and HeLa cells. Cytotoxicity of the pDNA complexes was assessed by Alamar blue assay. Complexes obtained through electrostatic association of the S4(13)-PV cell penetrating peptide with plasmid DNA are able to very effi...Continue Reading

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Mar 23, 2011·Pharmaceutical Research·Ethlinn V B van GaalEnrico Mastrobattista
May 19, 2012·Expert Opinion on Drug Delivery·Taavi LehtoÜlo Langel
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Apr 7, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sara TrabuloMaria C Pedroso de Lima
Mar 30, 2010·Pharmaceuticals·Sara TrabuloMaria C Pedroso De Lima
Apr 28, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wei QuXian-Zheng Zhang
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