PMID: 9419818Jan 7, 1998Paper

S49 cells endogenously express subtype 2 somatostatin receptors which couple to increase protein tyrosine phosphatase activity in membranes and down-regulate Raf-1 activity in situ

Cellular Signalling
P DentT W Sturgill

Abstract

S49 cells expressed type 2 somatostatin receptors (sstr2) by immunoblotting. Analysis by reverse transcription and polymerase chain reaction (RT-PCR) methodologies showed that S49 cells express predominantly sstr2A and sstr2B mRNAs; other subtypes were either not detected, in the case of sstr1, sstr3, sstr4, or variably detected, in the case of sstr5. No mutations were present in S49 cells at codon 12, 13, or 61 of the N-, K-, or H-ras genes. Nevertheless, randomly growing S49 cells contained Raf-1 activity by specific immune complex kinase assays. Treatment of S49 cells with somatostatin transiently inactivated the basal activity of Raf-1, but not that of B-Raf. Addition of somatostatin plus guanyl-5'-yl imidodiphosphate (GMPPNP) to S49 membranes stimulated PTPase activity. The concentration dependence for stimulation of PTPase activity correlated with high affinity binding of [125I-Tyr11]somatostatin-14. Both the effect of somatostatin to stimulate PTPase activity and to inactivate Raf-1 were abrogated by PTx. PTPase activity stimulated by somatostatin plus GMPPNP was recovered in a peak of high apparent M(r) (670,000) after solubilisation with Triton X-100 and Superose 6 chromatography. Furthermore, addition of activated, br...Continue Reading

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Citations

Nov 5, 2013·Trends in Pharmacological Sciences·Jean Claude Reubi, Agnes Schonbrunn
Sep 28, 2012·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·A O Shpakov
Jun 24, 2010·World Journal of Gastroenterology : WJG·Jonathan Strosberg, Larry Kvols
Oct 5, 2007·Molecular and Cellular Endocrinology·Tullio Florio
Aug 28, 2007·Pharmacology & Therapeutics·Davide Cervia, Paola Bagnoli
Jul 16, 2010·Journal of Cellular and Molecular Medicine·Mehtap CakirAshley Grossman
May 11, 2001·Neuropeptides·Z Csaba, P Dournaud
Aug 12, 2014·PloS One·Slávka KaščákováMartin P van Hagen

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