SAAV2152 is a single-stranded DNA binding protein: the third SSB in Staphylococcus aureus

Oncotarget
Yen-Hua Huang, Cheng-Yang Huang

Abstract

Single-stranded DNA-binding proteins (SSBs) play crucial roles in DNA replication, repair, and recombination. Unlike E. coli, which contains only one type of SSB (EcSSB), some bacteria have two paralogous SSBs, namely, SsbA and SsbB. In this study, we found the third SSB-like protein in Staphylococcus aureus, SAAV2152, which was designated as SaSsbC. SaSsbC is a protein of 131 amino acids and shares 38%, 36%, and 33% sequence identity to SaSsbB, SaSsbA, and EcSSB, respectively. Gene map analysis showed that unlike the E. coli ssb gene, which is adjacent to uvrA gene, the S. aureus ssb gene SAAV2152 is flanked by the putative SceD, the putative YwpF, and fabZ genes. A homology model showed that SaSsbC consists of the classic oligonucleotide/oligosaccharide-binding fold at the N-terminus. At the C-terminus, SaSsbC did not exhibit sequence similarity to that of EcSSB. Electrophoretic mobility shift analysis showed that SaSsbC formed a single complex with ssDNA of different lengths. Mutational analysis revealed that Tyr36, Tyr47, Phe53, and Tyr81 in SaSsbC are at positions that structurally correspond to the important residues of EcSSB for binding to ssDNA and are also critical for SaSsbC to bind ssDNA. Unlike EcSSB, which can stim...Continue Reading

References

Jan 1, 1994·Annual Review of Biochemistry·T M Lohman, M E Ferrari
Dec 30, 1999·Journal of Bacteriology·S J Sandler, K J Marians
Jan 14, 2000·Nature Structural Biology·G WaksmanJ M Carazo
Apr 8, 2000·Trends in Biochemical Sciences·K J Marians
Jun 11, 2002·Annual Review of Nutrition·Julie A Ross, Christine M Kasum
Sep 24, 2004·The Journal of Biological Chemistry·Jyung-Hurng LiuChwan-Deng Hsiao
Dec 4, 2004·Nucleic Acids Research·Chris J Cadman, Peter McGlynn
Sep 29, 2005·The Journal of Biological Chemistry·Chris J CadmanPeter McGlynn
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Dec 15, 2007·Medicinal Research Reviews·Florence TeilletXavier Ronot
Apr 17, 2008·Nature Reviews. Molecular Cell Biology·Timothy M LohmanColin G Wu
Oct 22, 2008·Critical Reviews in Biochemistry and Molecular Biology·Robert D SheredaJames L Keck
Apr 16, 2009·Critical Reviews in Biochemistry and Molecular Biology·Derek J RichardKum Kum Khanna
Apr 3, 2010·The Journal of Biological Chemistry·Alexander G KozlovTimothy M Lohman
Jan 7, 2011·The Protein Journal·Cheng-Chieh Chen, Cheng-Yang Huang
Jan 29, 2011·Nature·Anil KoulKoen Andries
Apr 27, 2011·FEMS Microbiology Reviews·Silvia AyoraJuan C Alonso
Sep 4, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yen-Hua HuangCheng-Yang Huang
Oct 24, 2012·Journal of Biomedicine & Biotechnology·Hsin-Hsien Lin, Cheng-Yang Huang
Dec 25, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nobuhiro KoyamaHiroshi Tomoda
Mar 29, 2013·PloS One·Aimee H MarceauJames L Keck
Jul 5, 2013·Structure·Thayne H DickeyDeborah S Wuttke
Aug 28, 2014·BioMed Research International·Yen-Hua Huang, Cheng-Yang Huang
Apr 22, 2015·The Protein Journal·Yen-Hua HuangCheng-Yang Huang
Jul 28, 2016·Tuberculosis·Amandeep SinghNagasuma Chandra
Sep 10, 2016·The Journal of Antimicrobial Chemotherapy·Jason G GlanzerGreg G Oakley
Sep 24, 2016·Journal of Structural Biology·Amandeep SinghM Vijayan

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Citations

Oct 13, 2019·Biochemical and Biophysical Research Communications·Yen-Hua HuangCheng-Yang Huang
Feb 17, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·J R Aguirre-SanchezC Chaidez-Quiroz

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Methods Mentioned

BETA
gel filtration
Gel
electrophoresis
PCR

Software Mentioned

SWISS
CLUSTALW2
ConSurf
SmartBlast
MODEL
RPS
BLAST

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