Saccharated colloidal iron enhances lipopolysaccharide-induced nitric oxide production in vivo

Free Radical Biology & Medicine
Akira I HidaShigeru Okada

Abstract

We investigated the effects of iron on the production of nitric oxide (NO), inducible NO synthase (iNOS), and plasma cytokines induced by lipopolysaccharide (LPS) in vivo. Male Wistar rats were preloaded with a single intravenous injection of saccharated colloidal iron (Fesin, 70 mg iron/kg body weight) or normal saline as a control, and then given an intraperitoneal injection of LPS (5.0 mg/kg body weight). Rats, preloaded with iron, had evidence of both iron deposition and strong iNOS induction in liver Kupffer cells upon injection of LPS; phagocytic cells in the spleen and lung had similar findings. LPS-induced NO production in iron-preloaded rats was significantly higher than control rats as accessed by NO-hemoglobin levels measured by ESR (electron spin resonance) and NOx (nitrate plus nitrite) levels. Western blot analysis showed that iron preloading significantly enhanced LPS-induced iNOS induction in the liver, but not in the spleen or lung. LPS-induced plasma levels of IL-6, IL-1beta, and TNF-alpha were also significantly higher in iron-preloaded rats as shown by ELISA, but IFN-gamma levels were unchanged. We conclude that colloidal-iron phagocytosed by liver Kupffer cells enhanced LPS-induced NO production in vivo, iN...Continue Reading

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Citations

Mar 31, 2004·Molecular Aspects of Medicine·Monica GalleanoSusana Puntarulo
Jun 2, 2005·Nitric Oxide : Biology and Chemistry·Pamela CornejoVirginia Fernández
Sep 11, 2012·Journal of Clinical Biochemistry and Nutrition·Yasumasa OkazakiShinya Toyokuni
Apr 5, 2012·Ultrastructural Pathology·Heba M SaadeldienMahmoud Rezk Abdelwahed Hussein
Aug 17, 2005·Molecular Aspects of Medicine·Susana Puntarulo
Oct 19, 2010·International Journal of Pharmaceutics·S F MedeirosA Elaissari
Oct 19, 2011·Toxicologic Pathology·Ivan RousseauSusana Puntarulo
Jun 25, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·De-Xiang XuWei Wei

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