Saccharomyces boulardii enhances N-terminal peptide hydrolysis in suckling rat small intestine by endoluminal release of a zinc-binding metalloprotease

Pediatric Research
Jean-Paul ButsS Marandi

Abstract

Saccharomyces boulardii (S. boulardii), a biotherapeutic agent effective in acute and chronic enterocolopathies, produces trophic intestinal effects at least in part mediated by the endoluminal release of polyamines. However, the effects of the yeast on peptide hydrolysis have not yet been studied. The objectives of this study were to assess in suckling rats the endoluminal and mucosal aminopeptidase activities in response to S. boulardii treatment and to analyze their related mechanisms. Peptidase activities were assayed on yeast cells by using several L-amino acid-p-nitroanilide substrates in the pH range of 2 to 10. A marked hydrolytic activity was found for L-leucine-p-nitroanilide that peaked at pH = 8 (K(m) = 0.334 mM, V(max) = 44.7 micromol.min(-1).g(-1) protein). N-terminal peptide hydrolysis was confirmed using as substrate L-Leu-Gly-Gly (K(m) = 4.71 mM, V(max) = 18.08 micromol.min(-1).g(-1) protein). Enzyme reactions were inhibited in the presence of 1 mM Zn(2+). Oral treatment of sucklings with S. boulardii significantly enhanced jejunal and ileal mucosal leucine-aminopeptidase activities by 24 and 34%, respectively, over controls. In concordance, aminopeptidase activity was enhanced in jejunal and ileal endoluminal ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 15, 1990·Biochemical and Biophysical Research Communications·J Z HaeggströmB Samuelsson
Sep 15, 1973·Biochimica Et Biophysica Acta·S MarouxJ Baratti
Jan 1, 1980·Biochimica Et Biophysica Acta·J B McClellan, C W Garner
Jan 1, 1982·Journal of Pediatric Gastroenterology and Nutrition·G F Vaeth, S J Henning
Jan 1, 1995·Methods in Enzymology·N D Rawlings, A J Barrett
May 1, 1993·Journal of Pediatric Gastroenterology and Nutrition·J P ButsM Delmee

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Citations

Apr 1, 2010·Nutrients·Saloomeh Moslehi-JenabianLene Jespersen
Jul 14, 2006·Digestive Diseases and Sciences·Jean-Paul Buts, Nadine De Keyser
Dec 20, 2008·European Journal of Pediatrics·Yvan VandenplasHania Szajewska
Aug 9, 2005·Gastroenterology Clinics of North America·Jean-Paul Buts, Paul Bernasconi
Sep 9, 2008·Scandinavian Journal of Gastroenterology·Jean-Paul ButsNadine De Keyser
Mar 17, 2012·Therapeutic Advances in Gastroenterology·Theodoros Kelesidis, Charalabos Pothoulakis
Dec 5, 2009·Scandinavian Journal of Gastroenterology·Jean-Paul Buts, Nadine De Keyser
Mar 23, 2006·Annals of the Royal College of Surgeons of England·Mehmet Faruk GeyikOmer Faruk Kokoglu
Aug 26, 2016·Critical Reviews in Microbiology·Amir SaberAhmad Yari Khosroushahi
Jul 19, 2020·Annual Review of Nutrition·William D FiersIliyan D Iliev
Jun 10, 2020·Journal of Fungi·Pedro PaisMiguel C Teixeira

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