Saccharomyces boulardii protease inhibits Clostridium difficile toxin A effects in the rat ileum.

Infection and Immunity
I CastagliuoloC Pothoulakis

Abstract

Saccharomyces boulardii, a nonpathogenic yeast, is effective in treating some patients with Clostridium difficile diarrhea and colitis. We have previously reported that S. boulardii inhibits rat ileal secretion in response to C. difficile toxin A possibly by releasing a protease that digests the intestinal receptor for this toxin (C. Pothoulakis, C. P. Kelly, M. A. Joshi, N. Gao, C. J. O'Keane, I. Castagliuolo, and J. T. LaMont, Gastroenterology 104: 1108-1115, 1993). The aim of this study was to purify and characterize this protease. S. boulardii protease was partially purified by gel filtration on Sephadex G-50 and octyl-Sepharose. The effect of S. boulardii protease on rat ileal secretion, epithelial permeability, and morphology in response to toxin A was examined in rat ileal loops in vivo. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified S. boulardii protease revealed a major band at 54 kDa. Pretreatment of rat ileal brush border (BB) membranes with partially purified protease reduced specific toxin A receptor binding (by 26%). Partially purified protease digested the toxin A molecule and significantly reduced its binding to BB membranes in vitro (by 42%). Preincubation of toxin A with S. boulardii...Continue Reading

References

Mar 9, 1978·The New England Journal of Medicine·J G BartlettA B Onderdonk
Nov 1, 1977·The Journal of Infectious Diseases·J G BartlettD L Kasper
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Feb 1, 1990·Infection and Immunity·C H DoveJ L Johnson
Nov 1, 1989·Gastroenterology·G TriadafilopoulosJ T Lamont
Jan 26, 1989·The New England Journal of Medicine·L V McFarlandW E Stamm
Aug 1, 1987·Gastroenterology·G TriadafilopoulosJ T LaMont
Nov 1, 1986·Canadian Journal of Microbiology·G CorthierR Ducluzeau
Jul 1, 1971·Analytical Biochemistry·J S RothE Wierbicki
Oct 1, 1984·Antimicrobial Agents and Chemotherapy·R D Toothaker, G W Elmer
Mar 1, 1982·Infection and Immunity·N M SullivanT D Wilkins
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·C PothoulakisS E Leeman
May 1, 1995·The Journal of Clinical Investigation·M RieglerJ T LaMont
Jan 27, 1994·The New England Journal of Medicine·C P KellyJ T LaMont
May 1, 1993·Journal of Pediatric Gastroenterology and Nutrition·J P ButsM Delmee
Sep 1, 1993·Gastroenterology·C PothoulakisD Rachmilewitz
Jan 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·L V McFarland

❮ Previous
Next ❯

Citations

Apr 20, 2002·Current Opinion in Infectious Diseases·Subhas Banerjee, J. Thomas Lamont
Oct 7, 2004·Canadian Journal of Microbiology·Juliana L R FiettoIeso M Castro
May 13, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Raoul Herbrecht, Yasmine Nivoix
Sep 20, 2008·The Journal of General and Applied Microbiology·Sandrine GraffChristine Charrueau
May 7, 2009·FEMS Microbiology Letters·Peter I DuncanIrène Corthésy-Theulaz
Jul 31, 2010·Gut Microbes·Carissa M Thomas, James Versalovic
Dec 7, 2010·Applied and Environmental Microbiology·Lucie Etienne-MesminStéphanie Blanquet-Diot
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·P Frey-KlettA Sarniguet
Mar 17, 2012·Therapeutic Advances in Gastroenterology·Theodoros Kelesidis, Charalabos Pothoulakis
Dec 12, 2012·The Journal of Dairy Research·Patricia Araceli BollaGraciela Liliana De Antoni
Jan 30, 2014·Current Rheumatology Reports·Hester EppingaH Bing Thio
Jul 6, 2014·Current Gastroenterology Reports·Lindsay M UrbenStephen A McClave
Aug 26, 2016·Critical Reviews in Microbiology·Amir SaberAhmad Yari Khosroushahi
Aug 21, 2019·Journal of Applied Microbiology·M M Y ElghandourA Z M Salem
Sep 27, 2019·Gut and Liver·Ningning YouJunping Shi
Oct 13, 1998·Alimentary Pharmacology & Therapeutics·S J Lewis, A R Freedman
Aug 15, 2003·World Journal of Gastroenterology : WJG·Fariborz Mansour-GhanaeiAfshin Shafaghi
Sep 5, 2007·Alimentary Pharmacology & Therapeutics·D CzeruckaP Rampal
Mar 30, 2011·Archivum Immunologiae Et Therapiae Experimentalis·Johannes StephaniJan Hendrik Niess
Feb 22, 2012·Current Opinion in Critical Care·Lee E MorrowMark A Malesker
Feb 16, 2019·Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine·Hayk Minasyan
Apr 24, 2020·Mediators of Inflammation·Voon Kin ChinMaha Abdullah
Jun 11, 2020·Expert Review of Anti-infective Therapy·Dianbin LiuYanxia Wei
Dec 20, 2008·European Journal of Pediatrics·Yvan VandenplasHania Szajewska
Nov 23, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·X WuK Jacobson
Oct 22, 2005·Digestive Diseases and Sciences·Philippe GirardJean-Marie Gillardin
Apr 1, 2010·Nutrients·Saloomeh Moslehi-JenabianLene Jespersen
Jan 15, 2008·Clinical and Experimental Immunology·M SchleeK Fellermann
May 29, 2009·FEMS Yeast Research·Gilzeane dos Santos Sant'AnaIeso de Miranda Castro
Oct 14, 2011·Journal of Clinical Gastroenterology·Xi Na, Ciaran Kelly
May 5, 2012·Gut Microbes·Emma L BestMark H Wilcox
Apr 25, 2013·The Journal of Dairy Research·Fernando M TrejoPablo F Pérez

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.