Saccharomyces cerevisiae--a model to uncover molecular mechanisms for yeast biofilm biology

FEMS Immunology and Medical Microbiology
Rasmus K BojsenBirgitte Regenberg

Abstract

Microbial biofilms can be defined as multi-cellular aggregates adhering to a surface and embedded in an extracellular matrix (ECM). The nonpathogenic yeast, Saccharomyces cerevisiae, follows the common traits of microbial biofilms with cell-cell and cell-surface adhesion. S. cerevisiae is shown to produce an ECM and respond to quorum sensing, and multi-cellular aggregates have lowered susceptibility to antifungals. Adhesion is mediated by a family of cell surface proteins of which Flo11 has been shown to be essential for biofilm development. FLO11 expression is regulated via a number of regulatory pathways including the protein kinase A and a mitogen-activated protein kinase pathway. Advanced genetic tools and resources have been developed for S. cerevisiae including a deletion mutant-strain collection in a biofilm-forming strain background and GFP-fusion protein collections. Furthermore, S. cerevisiae biofilm is well applied for confocal laser scanning microscopy and fluorophore tagging of proteins, DNA and RNA. These techniques can be used to uncover the molecular mechanisms for biofilm development, drug resistance and for the study of molecular interactions, cell response to environmental cues, cell-to-cell variation and nic...Continue Reading

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Citations

Feb 12, 2013·Applied Microbiology and Biotechnology·J A VallejoT G Villa
Apr 17, 2014·Journal of Biosciences·Dominika M Wloch-Salamon
Mar 5, 2013·Fungal Genetics and Biology : FG & B·Asier GonzálezJoaquín Ariño
Mar 15, 2015·Journal of Environmental Sciences (China)·Jinxue LuoScott A Rice
Nov 4, 2016·Proceedings. Biological Sciences·Birgitte RegenbergJacobus J Boomsma
Jan 1, 2015·Journal of the Royal Society, Interface·Kyle NowlinDennis LaJeunesse
Oct 31, 2018·Journal of Fungi·Ronnie G Willaert
Sep 6, 2014·Genetics·Colin A ChavelPaul J Cullen
Feb 15, 2020·NPJ Biofilms and Microbiomes·Phu Van NguyenZdena Palková
Apr 21, 2017·Frontiers in Physiology·Beatriz GonzálezMaría Jesús Torija
Feb 16, 2019·Frontiers in Microbiology·Giorgia PerpetuiniRosanna Tofalo
Aug 11, 2020·World Journal of Microbiology & Biotechnology·Giacomo ZaraSeverino Zara

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