Saccharomyces cerevisiae and Zygosaccharomyces mellis exhibit different hyperosmotic shock responses

Yeast
Jannik Vindeløv, N Arneborg

Abstract

The effect of hyperosmotic shock on cell volume, vacuole volume, and intracellular pH (pH(i)) of individual cells of Saccharomyces cerevisiae and Zygosaccharomyces mellis was investigated. After transfer from a high water activity (a(w)) medium to low a(w) media, the growth latency periods of Z. mellis were shorter than those of S. cerevisiae. These results demonstrate that Z. mellis manages hyperosmotic shock better than S. cerevisiae. As a response to acute hyperosmotic shock, i.e. the first minute of perfusion with hypertonic buffers, the vacuoles shrank and the pH(i) decreased in both yeasts. Furthermore, in the presence of glucose, vacuole shrinkage and intracellular acidification were more pronounced in S. cerevisiae than in Z. mellis. These results may be explained by the fact that the S. cerevisiae cells shrank more than the Z. mellis cells as a response to acute hyperosmotic shock. In the presence of glucose, the vacuoles and the cells of both S. cerevisiae and Z. mellis shrank simultaneously and in proportion to a minimum level during acute hyperosmotic shock, and remained constant at this level throughout the experiment (11 min). These results indicate that vacuoles do not act as water reserves in yeasts after acute ...Continue Reading

References

Jan 1, 1979·Basic Life Sciences·A D Brown, M Edgley
Apr 1, 1975·The Journal of Applied Bacteriology·D Rose
Jan 1, 1992·Advances in Microbial Physiology·A Blomberg, L Adler
Jan 1, 1989·Methods in Cell Biology·G R BrightD L Taylor
Sep 1, 1987·Applied and Environmental Microbiology·R H ReedG M Gadd
Jul 1, 1986·Journal of General Microbiology·G J MorrisK J Clarke
Mar 31, 1993·Biochemical and Biophysical Research Communications·M Latterich, M D Watson
Jun 1, 1990·Applied and Environmental Microbiology·M H ZwieteringK van 't Riet

❮ Previous
Next ❯

Citations

Apr 30, 2011·Journal of the Royal Society, Interface·Thomas AaboNils Arneborg
Jun 10, 2008·Applied and Environmental Microbiology·Henrik Dam MortensenNils Arneborg
Jul 28, 2009·Applied and Environmental Microbiology·Miguel C TeixeiraIsabel Sá-Correia
Mar 5, 2005·Applied and Environmental Microbiology·Minoska ValliDiethard Mattanovich
May 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Xin-Liang ZhouYoshiro Saimi
Oct 7, 2004·Fungal Genetics and Biology : FG & B·Roger R LewAshley Garrill
Sep 24, 2004·Journal of Biotechnology·Diethard MattanovichMichael Sauer
Jul 12, 2002·Yeast
Jun 15, 2007·The Journal of Membrane Biology·Hélène SimoninPatrick Gervais
Oct 22, 2005·Applied Microbiology and Biotechnology·H D MortensenN Arneborg
Mar 11, 2009·The Biochemical Journal·Stephen K DoveRobert H Michell
Mar 7, 2017·Frontiers in Microbiology·Yu ZhaoHenrik Siegumfeldt
Jan 22, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Carlos SaldañaTeresa García-Gasca

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.