Saccharomyces cerevisiae expressing Gp43 protects mice against Paracoccidioides brasiliensis infection

PloS One
Mariana Aprigio Assis-MarquesPaulo Sergio Rodrigues Coelho

Abstract

The dimorphic fungus Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis (PCM). It is believed that approximately 10 million people are infected with the fungus and approximately 2% will eventually develop the disease. Unlike viral and bacterial diseases, fungal diseases are the ones against which there is no commercially available vaccine. Saccharomyces cerevisiae may be a suitable vehicle for immunization against fungal infections, as they require the stimulation of different arms of the immune response. Here we evaluated the efficacy of immunizing mice against PCM by using S. cerevisiae yeast expressing gp43. When challenged by inoculation of P. brasiliensis yeasts, immunized animals showed a protective profile in three different assays. Their lung parenchyma was significantly preserved, exhibiting fewer granulomas with fewer fungal cells than found in non-immunized mice. Fungal burden was reduced in the lung and spleen of immunized mice, and both organs contained higher levels of IL-12 and IFN-γ compared to those of non-vaccinated mice, a finding that suggests the occurrence of Th1 immunity. Taken together, our results indicate that the recombinant yeast vaccine represents a new strategy to conf...Continue Reading

References

Feb 28, 1979·Mycopathologia·V L CalichC R Paula
Jan 1, 1992·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·L E CanoA Restrepo
Oct 1, 1991·Immunology Today·P Scott, S H Kaufmann
Jul 1, 1991·American Journal of Respiratory Cell and Molecular Biology·C H ModyG B Toews
Feb 1, 1987·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·M Franco
Dec 1, 1985·Mycopathologia·S S KashinoL M Singer-Vermes
Apr 1, 1993·Clinical Microbiology Reviews·E BrummerA Restrepo
Jul 1, 1997·Infection and Immunity·R Allendoerfer, G S Deepe
Sep 25, 1999·The American Journal of Tropical Medicine and Hygiene·M H BlottaZ P Camargo
Feb 6, 2002·Trends in Microbiology·M Ines Borges-WalmsleyAdrian R Walmsley
Dec 3, 2005·Genes & Development·Daniel M GelperinElizabeth J Grayhack
Mar 13, 2008·PLoS Neglected Tropical Diseases·Leandro Licursi de OliveiraAdemilson Panunto-Castelo
Jul 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Elizabeth K WansleyJames W Hodge
Sep 9, 2008·Mycopathologia·Célia Maria de Almeida SoaresVishnu Chaturvedi
Feb 21, 2009·PloS One·Luciana Pereira RuasMaria-Cristina Roque-Barreira
May 26, 2009·Vaccine·Javier CapillaDavid A Stevens
Jan 5, 2010·Vaccine·Alice M RibeiroMaria Sueli S Felipe
Jul 9, 2010·Medical Mycology·David A StevensMin Liu
Mar 6, 2012·PLoS Neglected Tropical Diseases·Glauce M G RittnerLuiz R Travassos
May 23, 2012·Cell Host & Microbe·René M Roy, Bruce S Klein
Jun 13, 2012·Immunological Investigations·Min LiuDavid A Stevens
Aug 17, 2012·Human Vaccines & Immunotherapeutics·Luiz R Travassos, Carlos P Taborda
Aug 15, 2014·Mycopathologia·Tanya MajumderDavid A Stevens
Nov 2, 2014·Frontiers in Immunology·Monica BorghiLuigina Romani

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Citations

Nov 18, 2015·Immunological Investigations·Kausik Datta, Mawieh Hamad
Nov 5, 2016·ACS Applied Materials & Interfaces·Yadveer S GrewalMatt Trau
Nov 26, 2015·Memórias do Instituto Oswaldo Cruz·Natasha P Medici, Maurizio Del Poeta
Sep 28, 2018·PLoS Neglected Tropical Diseases·Daniele Gonçalves CastilhoWagner Luiz Batista
Jan 23, 2019·FEMS Yeast Research·Ravinder Kumar, Piyush Kumar
Nov 27, 2018·Current Molecular Pharmacology·Manaf AlMatarFatih Köksal
Nov 7, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Sanam NamiHamid Morovati
Oct 23, 2018·Pharmacology & Therapeutics·André Moraes NicolaMaria Sueli Felipe

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Methods Mentioned

BETA
PCR
ELISA

Software Mentioned

GraphPad Prism5
KS

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