Saccharomyces cerevisiae G1 cyclins differ in their intrinsic functional specificities.

Molecular and Cellular Biology
K LevineF R Cross

Abstract

The three budding yeast CLN genes appear to be functionally redundant for cell cycle Start: any single CLN gene is sufficient to promote Start, while the cln1 cln2 cln3 triple mutant is Start defective and inviable. Both quantitative and apparently qualitative differences between CLN genes have been reported, but available data do not in general allow distinction between qualitative functional differences as opposed to simply quantitative differences in expression or function. To determine if there are intrinsic qualitative differences between Cln proteins, we compared CLN2, CLN3, and crippled (but still partially active) CLN2 genes in a range of assays that differentiate genetically between CLN2 and CLN3. The results suggest that different potencies of Cln2, Cln3, and Cln2 mutants in functional assays cannot be accounted for by a simple quantitative model for their action, since Cln3 is at least as active as Cln2 and much more active than the Cln2 mutants in driving Swi4/Swi6 cell cycle box (SCB)-regulated transcription and cell cycle initiation in cln1 cln2 cln3 bck2 strains, but Cln3 has little or no activity in other assays in which Cln2 and the Cln2 mutants function. Differences in Cln protein abundance are unlikely to acc...Continue Reading

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Citations

Nov 3, 2007·IEEE/ACM Transactions on Computational Biology and Bioinformatics·Ignacio PonzoniDavid Glass
Aug 15, 2012·Cell Cycle·Inma Quilis, Juan Carlos Igual
Apr 29, 2011·BMC Bioinformatics·Cristian A GalloIgnacio Ponzoni
Aug 13, 1999·Molecular Cell·F R CrossM D Jacobson
Jun 23, 2011·PloS One·Catherine Oikonomou, Frederick R Cross
Aug 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tracy L LaabsWarren Heideman
Jul 31, 1999·Cell·J M Roberts
Jan 26, 2002·Molecular Biology of the Cell·Frederick R CrossMartha Klovstad
Feb 26, 2000·Molecular Biology of the Cell·C Martín-CastellanosS Moreno
Jun 26, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kedar PurnapatreSaul M Honigberg
Jan 25, 2007·Nature Reviews. Molecular Cell Biology·Joanna Bloom, Frederick R Cross
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Sep 17, 1998·The Journal of Biological Chemistry·L J Oehlen, F R Cross
Nov 23, 2006·Molecular and Cellular Biology·Joanna Bloom, Frederick R Cross
May 25, 2002·Molecular and Cellular Biology·Herman WijnenBruce Futcher
Jun 10, 2000·Molecular and Cellular Biology·M D JacobsonF R Cross
Jun 10, 2000·Molecular and Cellular Biology·F R Cross, M D Jacobson
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