Saccharomyces cerevisiae HMO1 interacts with TFIID and participates in start site selection by RNA polymerase II

Nucleic Acids Research
Koji KasaharaTetsuro Kokubo

Abstract

Saccharomyces cerevisiae HMO1, a high mobility group B (HMGB) protein, associates with the rRNA locus and with the promoters of many ribosomal protein genes (RPGs). Here, the Sos recruitment system was used to show that HMO1 interacts with TBP and the N-terminal domain (TAND) of TAF1, which are integral components of TFIID. Biochemical studies revealed that HMO1 copurifies with TFIID and directly interacts with TBP but not with TAND. Deletion of HMO1 (Deltahmo1) causes a severe cold-sensitive growth defect and decreases transcription of some TAND-dependent genes. Deltahmo1 also affects TFIID occupancy at some RPG promoters in a promoter-specific manner. Interestingly, over-expression of HMO1 delays colony formation of taf1 mutants lacking TAND (taf1DeltaTAND), but not of the wild-type strain, indicating a functional link between HMO1 and TAND. Furthermore, Deltahmo1 exhibits synthetic growth defects in some spt15 (TBP) and toa1 (TFIIA) mutants while it rescues growth defects of some sua7 (TFIIB) mutants. Importantly, Deltahmo1 causes an upstream shift in transcriptional start sites of RPS5, RPS16A, RPL23B, RPL27B and RPL32, but not of RPS31, RPL10, TEF2 and ADH1, indicating that HMO1 may participate in start site selection of a...Continue Reading

References

Jan 2, 1992·Gene·T W ChristiansonP Hieter
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·Z W Sun, M Hampsey
Jun 1, 1995·Genes & Development·B M ShykindP A Sharp
Feb 15, 1995·Genes & Development·M W HullN A Woychik
Jul 25, 1993·Nucleic Acids Research·C M ReadD G Norman
Dec 27, 1996·The Journal of Biological Chemistry·J LuS J Brill
Jun 1, 1997·Molecular and Cellular Biology·A AronheimM Karin
Feb 3, 1998·Molecular and Cellular Biology·T KokuboY Nakatani
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·F Winston, P Sudarsanam
Jun 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·T KotaniT Kokubo
Mar 14, 2001·Trends in Biochemical Sciences·M Bustin
Mar 14, 2001·Trends in Biochemical Sciences·J O Thomas, A A Travers
Apr 3, 2001·Proceedings of the National Academy of Sciences of the United States of America·T ItoY Sakaki
Jun 22, 2001·The Journal of Biological Chemistry·C ChangT Kodadek
Sep 7, 2002·Molecular and Cellular Biology·Jennifer A FairleyStefan G E Roberts
Sep 21, 2002·Molecular and Cellular Biology·Nevan J KroganJack F Greenblatt
Oct 11, 2002·The EMBO Journal·Olivier GadalPierre Thuriaux
Apr 4, 2003·Current Opinion in Genetics & Development·Alessandra Agresti, Marco E Bianchi
Oct 3, 2003·The Journal of Biological Chemistry·Lynn M ZieglerAlfred S Ponticelli

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Citations

Nov 21, 2014·Bioscience, Biotechnology, and Biochemistry·Ayako HigashinoKoji Kasahara
Jun 12, 2013·Experimental Cell Research·Koichi EguchiMasamitsu Yamaguchi
Dec 3, 2016·Microbiology and Molecular Biology Reviews : MMBR·Arvind Panday, Anne Grove
May 16, 2008·The EMBO Journal·Raghuvir S TomarJoseph C Reese
Sep 27, 2019·Proceedings of the National Academy of Sciences of the United States of America·Francisco Aresta-BrancoLuisa M Figueiredo
Apr 26, 2019·PloS One·Eszter BokorZsuzsanna Hamari
Nov 20, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Koji KasaharaTetsuro Kokubo
Jul 10, 2021·Biochemical Society Transactions·David ShoreBenjamin Albert
Sep 20, 2015·Genes & Development·Rohit RejaB Franklin Pugh

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Methods Mentioned

BETA
PCR
pulldown
Two-Hybrid
immunoprecipitation
ChIP

Software Mentioned

SAGA

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