PMID: 11911362Mar 26, 2002Paper

Saccharomyces cerevisiae nucleolar protein Nop7p is necessary for biogenesis of 60S ribosomal subunits

RNA
Cynthia C AdamsJ L Woolford

Abstract

To identify new gene products that participate in ribosome biogenesis, we carried out a screen for mutations that result in lethality in combination with mutations in DRS1, a Saccharomyces cerevisiae nucleolar DEAD-box protein required for synthesis of 60S ribosomal subunits. We identified the gene NOP7that encodes an essential protein. The temperature-sensitive nop7-1 mutation or metabolic depletion of Nop7p results in a deficiency of 60S ribosomal subunits and accumulation of halfmer polyribosomes. Analysis of pre-rRNA processing indicates that nop7 mutants exhibit a delay in processing of 27S pre-rRNA to mature 25S rRNA and decreased accumulation of 25S rRNA. Thus Nop7p, like Drs1p, is required for essential steps leading to synthesis of 60S ribosomal subunits. In addition, inactivation or depletion of Nop7p also affects processing at the A0, A1, and A2 sites, which may result from the association of Nop7p with 35S pre-rRNA in 90S pre-rRNPs. Nop7p is localized primarily in the nucleolus, where most steps in ribosome assembly occur. Nop7p is homologous to the zebrafish pescadillo protein necessary for embryonic development. The Nop7 protein contains the BRCT motif, a protein-protein interaction domain through which, for examp...Continue Reading

References

Dec 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·T L RipmasterJ L Woolford
Dec 1, 1990·The Journal of Cell Biology·M MoritzJ L Woolford
Mar 1, 1989·Genes & Development·M Caizergues-FerrerF Amaldi
Feb 21, 1966·Biochimica Et Biophysica Acta·H Wallace, M L Birnstiel
Aug 1, 1982·Cell·P Pierandrei-AmaldiF Amaldi
Jun 1, 1995·Trends in Genetics : TIG·V Doye, E C Hurt
Jul 1, 1996·Nature Genetics·E V KooninP Bork
Nov 3, 1998·The EMBO Journal·X ZhangP S Freemont
Mar 24, 1999·The Journal of Cell Biology·E GiordanoM Furia
May 14, 1999·Trends in Biochemical Sciences·J de la CruzP Linder
Nov 5, 1999·Trends in Biochemical Sciences·J R Warner
Feb 26, 2000·Annual Review of Genetics·J Venema, D Tollervey
Nov 10, 2000·Molecular Biology of the Cell·T Stage-ZimmermannP A Silver
Feb 15, 2001·Nature Structural Biology·B Schwer
Jun 1, 1958·Experimental Cell Research·T R ELSDALES SMITH
Jan 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·D D BROWN, J B GURDON

❮ Previous
Next ❯

Citations

Jul 28, 2006·Molecular Genetics and Genomics : MGG·Carl R UrbinatiRoy M Long
Sep 6, 2003·Gene·Micheline Fromont-RacineFranco Fasiolo
Jan 27, 2004·Molecular Biology of the Cell·Jens EilbrachtMarion S Schmidt-Zachmann
Jan 27, 2010·Nucleic Acids Research·Juliane MerlHerbert Tschochner
Dec 14, 2004·Eukaryotic Cell·Kara A BernsteinSusan J Baserga
Jun 6, 2007·Molecular and Cellular Biology·Pilar Martín-MarcosMercedes Tamame
Jun 13, 2012·Molecular and Cellular Biology·Reyes BabianoJesús de la Cruz
Jul 1, 2004·Molecular and Cellular Biology·Brigitte PertschyHelmut Bergler
Nov 17, 2005·Molecular and Cellular Biology·Tiffany D MilesJohn L Woolford
Jan 20, 2006·Genome Biology·Chad L MyersOlga G Troyanskaya
Apr 22, 2004·RNA·Edward W HorseyJohn L Woolford
Mar 3, 2012·PloS One·Timothy Simmons, Bruce Appel
Jun 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hunter B FraserMichael B Eisen
Sep 2, 2005·Chromosoma·Aaron A Van HooserRebecca Heald
Sep 6, 2014·Nucleic Acids Research·Minshi WangDimitri G Pestov
Dec 15, 2015·Nucleic Acids Research·Matthias ThomsIrmgard Sinning
Feb 5, 2013·Trends in Cell Biology·Katrin Karbstein
Jan 16, 2010·The American Journal of Pathology·Natalie TulchinAlvaro N A Monteiro
Mar 5, 2016·Cell Cycle·Leszek WawiórkaMarek Tchórzewski
Aug 6, 2013·The Plant Journal : for Cell and Molecular Biology·Hui Kyung ChoHyun-Sook Pai
Jul 21, 2011·Biology of the Cell·Aleksandra TeczaSusanne J Kühl
Oct 2, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jieping LiZongming Huang
Jan 30, 2013·Biochimica Et Biophysica Acta·Olga Rodríguez-GalánJesús de la Cruz
Dec 3, 2014·Plant Science : an International Journal of Experimental Plant Biology·Aris ZografidisKosmas Haralampidis
Jul 1, 2004·Molecular Cell·Yevgeniya R LapikDimitri G Pestov
Aug 31, 2007·Developmental Biology·Susanne GessertMichael Kühl
Jul 12, 2002·Yeast
Mar 13, 2003·Archives of Microbiology·Jeanette BrejningNils Arneborg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.