Saccharomyces cerevisiae possesses two functional homologues of Escherichia coli endonuclease III

Biochemistry
H J YouPaul W Doetsch

Abstract

We previously identified two distinct genes of Saccharomyces cerevisiae redoxyendonuclease (SCR1 and SCR2) which possess a high degree of sequence similarity to Escherichia coli endonuclease III [Augeri, L., Lee, Y. M., Barton, A. B., and Doetsch, P. W. (1997) Biochemistry 36, 721-729]. The proteins encoded by SCR1 and SCR2 were overexpressed in E. coli and purified to apparent homogeneity. Both proteins recognized and cleaved DNA substrates containing dihydrouracil, 2,6-diamino-4-hydroxy-5N-methylformamidopyrimidine (FaPy-7-MeGua), and abasic sites but not DNA substrates containing uracil or 8-oxoguanine. Purified Scr2, but not Scr1, possesses spectral properties which indicate the presence of an iron-sulfur center. Kinetic parameters for Scr1 and Scr2 were determined by using an oligonucleotide containing a single dihydrouracil. Analysis of the deduced amino acid sequences of Scr1 and Scr2 suggests that Scr2 bears an iron-sulfur motif, while Scr1 does not have this motif. However, Scr1 has a long, positively charged N-terminus that could be a mitochondrial transit sequence. Targeted gene disruption of SCR1 and SCR2 produced a double mutant that had no detectable enzymatic activity against the dihydrouracil-containing substrat...Continue Reading

References

Jan 1, 1987·Methods in Enzymology·G M WahlA R Kimmel
Jul 1, 1997·The Biochemical Journal·H E KrokanG Slupphaug

❮ Previous
Next ❯

Citations

Jan 4, 2003·Chembiochem : a European Journal of Chemical Biology·Sachdev S SidhuKurt Deshayes
Dec 29, 2010·Molecular Genetics and Genomics : MGG·Roland KlassenFriedhelm Meinhardt
Apr 25, 2012·Extremophiles : Life Under Extreme Conditions·Xiaoting HuaYuejin Hua
Nov 26, 2003·Mutation Research·Svein Bjelland, Erling Seeberg
Aug 1, 2000·Mutation Research·A Memisoglu, L Samson
Jun 12, 2002·Free Radical Biology & Medicine·Serge BoiteuxNathalie Guibourt
Jun 28, 2002·Free Radical Biology & Medicine·Susan S Wallace
Jan 17, 2004·Nucleic Acids Research·Holly MillerArthur P Grollman
Jun 1, 2005·Molecular and Cellular Biology·Nicole A DoudicanPaul W Doetsch
Jun 29, 2000·Annual Review of Biochemistry·A K McCulloughR S Lloyd
Aug 2, 2001·Biochemical and Biophysical Research Communications·J E KimM H Chung
Aug 29, 2003·International Journal of Radiation Biology·Q M ZhangS Yonei
Nov 28, 2015·The FEBS Journal·Carlos H Trasviña-ArenasLuis G Brieba
Nov 19, 2005·DNA Repair·Ratsavarinh VongsamphanhDindial Ramotar
Aug 2, 2005·Mutation Research·Miral Dizdaroglu
Mar 22, 2015·Mutation Research. Reviews in Mutation Research·Miral Dizdaroglu
Sep 8, 2017·Metallomics : Integrated Biometal Science·Sergi PuigMaría Teresa Martínez-Pastor
Aug 13, 2005·The Journal of Biological Chemistry·Takashi WatanabeJeffrey P Bond

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.