SACE_5599, a putative regulatory protein, is involved in morphological differentiation and erythromycin production in Saccharopolyspora erythraea

Microbial Cell Factories
Benjamin KirmGregor Kosec

Abstract

Erythromycin is a medically important antibiotic, biosynthesized by the actinomycete Saccharopolyspora erythraea. Genes encoding erythromycin biosynthesis are organized in a gene cluster, spanning over 60 kbp of DNA. Most often, gene clusters encoding biosynthesis of secondary metabolites contain regulatory genes. In contrast, the erythromycin gene cluster does not contain regulatory genes and regulation of its biosynthesis has therefore remained poorly understood, which has for a long time limited genetic engineering approaches for erythromycin yield improvement. We used a comparative proteomic approach to screen for potential regulatory proteins involved in erythromycin biosynthesis. We have identified a putative regulatory protein SACE_5599 which shows significantly higher levels of expression in an erythromycin high-producing strain, compared to the wild type S. erythraea strain. SACE_5599 is a member of an uncharacterized family of putative regulatory genes, located in several actinomycete biosynthetic gene clusters. Importantly, increased expression of SACE_5599 was observed in the complex fermentation medium and at controlled bioprocess conditions, simulating a high-yield industrial fermentation process in the bioreactor...Continue Reading

References

May 1, 1990·Journal of Bacteriology·J M WeberJ P DeWitt
Jul 20, 1999·Microbiology·A De Schrijver, R De Mot
Apr 12, 2001·Molecular Microbiology·M A ElliotB K Leskiw
Dec 4, 2001·Current Opinion in Microbiology·K F Chater
Feb 15, 2003·FEMS Microbiology Letters·Andreas RascherC Richard Hutchinson
Nov 24, 2004·Molecular Microbiology·George StratigopoulosEric Cundliffe
Apr 2, 2005·Current Opinion in Microbiology·Mervyn J Bibb
Oct 13, 2005·The FEBS Journal·Stephen F AltschulYi-Kuo Yu
Nov 28, 2007·Microbial Cell Factories·Clelia PeanoGianluca De Bellis
May 9, 2008·Nucleic Acids Research·Christian ColeGeoffrey J Barton
Aug 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Chinping ChngCamilla M Kao
May 19, 2009·Applied and Environmental Microbiology·Yukinori TanakaKozo Ochi
Mar 23, 2010·Current Opinion in Microbiology·Juan-Francisco Martín, Paloma Liras
May 26, 2011·Natural Product Reports·Gilles P van Wezel, Kenneth J McDowall
Nov 19, 2011·FEMS Microbiology Reviews·Joseph R McCormick, Klas Flärdh
Oct 23, 2012·BMC Microbiology·Dušan GoranovičHrvoje Petković

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Citations

May 6, 2014·Journal of Industrial Microbiology & Biotechnology·Panpan WuBuchang Zhang
Sep 14, 2015·Journal of Industrial Microbiology & Biotechnology·Richard H Baltz
Jan 1, 2015·Applied Microbiology and Biotechnology·Hang WuBuchang Zhang
Nov 12, 2018·Journal of Industrial Microbiology & Biotechnology·Je Won Park, Yeo Joon Yoon
Oct 21, 2018·Applied and Environmental Microbiology·Yurong XuBuchang Zhang

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Datasets Mentioned

BETA
ABE1441

Methods Mentioned

BETA
PCR
RNASeq
electrophoresis

Software Mentioned

BlastP
SAS
Primer Express
ClustalW2
STAT
MaxQuant

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