SACK-expanded hair follicle stem cells display asymmetric nuclear Lgr5 expression with non-random sister chromatid segregation.

Scientific Reports
Yang Hoon HuhJames L Sherley

Abstract

We investigated the properties of clonally-expanded mouse hair follicle stem cells (HF-SCs) in culture. The expansion method, suppression of asymmetric cell kinetics (SACK), is non-toxic and reversible, allowing evaluation of the cells' asymmetric production of differentiating progeny cells. A tight association was discovered between non-random sister chromatid segregation, a unique property of distributed stem cells (DSCs), like HF-SCs, and a recently described biomarker, Lgr5. We found that nuclear Lgr5 expression was limited to the HF-SC sister of asymmetric self-renewal divisions that retained non-randomly co-segregated chromosomes, which contain the oldest cellular DNA strands, called immortal DNA strands. This pattern-specific Lgr5 association poses a potential highly specific new biomarker for delineation of DSCs. The expanded HF-SCs also maintained the ability to make differentiated hair follicle cells spontaneously, as well as under conditions that induced cell differentiation. In future human cell studies, this capability would improve skin grafts and hair replacement therapies.

References

Jul 1, 1989·Japanese Journal of Cancer Research : Gann·T Kuroki, Y Murakami
Aug 12, 1999·The New England Journal of Medicine·R Paus, G Cotsarelis
Dec 19, 2002·Journal of Biomedicine & Biotechnology·Lakshmi RambhatlaJames L. Sherley
May 21, 2003·The Journal of Biological Chemistry·Benoit BoivinBruce G Allen
Jul 31, 2003·Biotechnology and Bioengineering·Hsuan-Shu LeeJames L Sherley
Dec 13, 2003·Science·Tudorita TumbarElaine Fuchs
Mar 17, 2004·Nature Biotechnology·Rebecca J MorrisGeorge Cotsarelis
Aug 24, 2005·The Journal of Cell Biology·Phillip KarpowiczDerek van der Kooy
Feb 25, 2006·Science·Athanasios Armakolas, Amar J S Klar
Jun 20, 2006·Current Topics in Developmental Biology·Jean-François Paré, James L Sherley
Jul 11, 2006·Annual Review of Cell and Developmental Biology·Cédric Blanpain, Elaine Fuchs
Jan 23, 2008·The Journal of Cell Biology·Elaine Fuchs
May 31, 2008·Journal of Tissue Engineering and Regenerative Medicine·Hyoungshin ParkGordana Vunjak-Novakovic
Aug 2, 2008·Molecular & Cellular Proteomics : MCP·Lisa BerglundMathias Uhlén
Oct 14, 2008·Nature Genetics·Viljar JaksRune Toftgård
Apr 4, 2009·Cerebral Cortex·Ji-Feng Fei, Wieland B Huttner
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sharon R PineCurtis C Harris
Dec 1, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Gregory W Charville, Thomas A Rando
Mar 31, 2011·Nature Communications·Marion EscobarCatherine Legraverend
Sep 10, 2011·Stem Cells·Yang Hoon Huh, James L Sherley

❮ Previous
Next ❯

Citations

May 18, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Sanjeev K Waghmare, Tudorita Tumbar
May 18, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Brendan Evano, Shahragim Tajbakhsh
Oct 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yang Hoon HuhJames L Sherley
Jun 16, 2012·BMC Cell Biology·Ratan K Choudhary, Anthony V Capuco
May 25, 2013·Seminars in Cell & Developmental Biology·Gregory W Charville, Thomas A Rando
Jan 1, 2013·Annual Review of Animal Biosciences·Anthony V Capuco, Steven E Ellis
Jan 7, 2016·Development·Michael B Schultz, David A Sinclair
Jul 6, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jette Lengefeld, Yves Barral

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy

Software Mentioned

NIH Image J

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.